基于高通量测序的实仓不同点位稻谷理化性质及真菌群落结构差异比较
Comparison of differences in physicochemical properties and fungal community structure of paddy at different positions in real warehouses using high-throughput sequencing
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摘要: 对实仓不同点位的稻谷进行为期12个月的跟踪监测,测定其水分含量、脂肪酸值和霉菌总数,并采用高通量测序技术解析真菌群落结构的变化规律。结果表明:随着储藏时间的延长,中心点位稻谷样品(CS、LTS、LTS2)和四周点位稻谷样品(SS、HTS、HTS2)的水分含量均有所下降,而脂肪酸值均有所升高,其中SS样品携带的霉菌总数高于同期的CS样品,而HTS2样品霉菌的生长繁殖受到抑制,总数低于同期的LTS2样品;四周点位稻谷样品的Chao1和Observed species指数均逐渐减小,Shannon和Simpson指数均先减小后增大,Pielou’s evenness指数均大于同期四周点位稻谷样品;中心点位稻谷样品的Chao1、Observed species、Shannon和Simpson指数均先增大后减小,且Good’s coverage指数同四周点位稻谷样品一样,均大于0.99。实仓不同点位稻谷样品的真菌群落被分为子囊菌门(Ascomycota)、担子菌门(Basidiomycota)、毛霉门(Mucoromycola)、油壶菌门(Olpidiomycota)和被孢霉门(Mortierellomycota)这5个主要菌门,且巨座壳属(Magnaporthe)等菌属及早熟副茎孢菌(Parastagonospora_ poae)等菌种在不同点位稻谷样品中呈现不同的变化趋势;所有点位稻谷样品共有的ASV/OTU数量为38个,其中拥有独特真菌数量最多的是LTS样品,拥有独特真菌数量最少的是SS样品;另外,主成分分析(PCA)显示四周点位稻谷样品分布相对分散,而中心点位稻谷样品分布相对集中。因此,相较于实仓的四周点位,中心点位可提供相对更稳定的微环境,更有利于粮情的稳定。Abstract: Paddy samples at different positions in real warehouses were monitored for 12 months to determine their moisture content, fatty acid value, and total mold count. The changes in fungal community structure were analyzed using high-throughput sequencing technology. The results showed that as storage time prolonged, the moisture content of paddy samples at center positions (CS, LTS, LTS2) and peripheral positions (SS, HTS, HTS2) decreased, while the fatty acid value increased. The total mold count of SS samples was higher than that of CS samples at the same time point, whereas mold growth and reproduction in HTS2 samples were inhibited, resulting in a lower total count compared to LTS2 samples at the same time point. For paddy samples at peripheral positions, the Chao1 and Observed species indices gradually decreased, while the Shannon and Simpson indices first decreased and then increased. The Pielou’s evenness index of paddy samples at center positions was higher than that of samples at peripheral positions at the corresponding time points. The Chao1, Observed species, Shannon, and Simpson indices ofpaddy samples at center positions first increased and then decreased, and the Good’s coverage index for all samples was greater than 0.99. The fungal communities in paddy samples from different positions were classified into five main phyla: Ascomycota, Basidiomycota, Mucoromycota, Olpidiomycota, and Mortierellomycota. Fungal genera such as Magnaporthe and species such as Parastagonospora_poae exhibited distinct variation trends in paddy samples from different positions. Furthermore, among all paddy samples, 38 ASV/OTU were shared. LTS samples had the highest number of unique fungi, while SS samples had the lowest. In addition, principal component analysis (PCA) revealed that paddy samples at peripheral positions were relatively dispersed in distribution, while those at center positions were relatively concentrated. This indicates that, compared to peripheral positions in real warehouses, center positions provide a more stable microenvironment, which is more favorable for maintaining grain conditions.
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Key words:
- paddy /
- storage /
- high-throughput sequencing /
- fungal community structure /
- grain condition
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