氧化铈抗氧化及抗光老化性能研究
Research on the antioxidant and anti-photoaging properties of cerium oxide
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摘要: 【目的】 系统评价氧化铈的抗氧化性能,并阐明其对长波紫外线(UVA)及模拟日光诱导的皮肤光老化损伤的保护效应。【方法】 通过DPPH·、O2-·及·OH自由基清除实验测定氧化铈的抗氧化性能,利用H2O2循环清除实验评估其可循环抗氧化性能,并分别以UVA与日光模拟器诱导人皮肤成纤维细胞(HFF-1)建立光老化模型,检测其细胞活力、Ⅰ型胶原蛋白(COL-Ⅰ)及Ⅲ型胶原蛋白(COL-Ⅲ)表达量。【结果】 氧化铈具有较好的抗氧化性能,质量浓度为6 mg/mL的氧化铈对DPPH·、O2-·及·OH 自由基的清除率分别为83.42%、30.29%和48.50%,且经3次H2O2循环处理后清除率仍保持90%以上,显示出较好的可循环抗氧化性能。与阴性对照组相比,质量浓度为0.078 mg/mL的氧化铈可将UVA诱导HFF-1的细胞活力提高38.78%,且在该质量浓度下,COL-Ⅰ表达量较阴性对照组提高了77.0倍。在模拟日光诱导损伤中,质量浓度为0.039 mg/mL的氧化铈可使COL-Ⅲ表达量提升至14.06 ng/mL,是阴性对照组的1.5倍。【结论】 氧化铈具有较好的可循环抗氧化性能,可通过上调 COL-Ⅰ与COL-Ⅲ表达量显著缓解UVA及模拟日光诱导HFF-1的光老化损伤,具备开发为新型抗氧化及抗光老化活性成分的潜力。Abstract: 【Objective】 To systematically evaluate the antioxidant properties of cerium oxide and elucidate its protective efficacy against UVA- and simulated sunlight-induced photoaging. 【Methods】 The antioxidant properties of cerium oxide were assessed through DPPH·, superoxide anion (O2-·), and hydroxyl radical (·OH) scavenging assays. Its recyclable antioxidant capacity was evaluated using an H2O2 recycling scavenging assay. In vitro photodamage models were established in human skin fibroblasts (HFF-1) using UVA irradiation and a solar simulator, followed by measurements of cellular viability and expression levels of type I (COL-Ⅰ) and type III (COL-Ⅲ) collagen. 【Results】 Cerium oxide exhibited significant antioxidant capacity. At a concentration of 6 mg/mL, it showed scavenging efficiencies of 83.42%, 30.29%, and 48.50% for DPPH·, O2-·, and ·OH radicals respectively. After three H2O2 recycling cycles, these efficiencies remained above 90%, confirming excellent recyclable antioxidant properties. Compared to the negative control, cerium oxide at 0.078 mg/mL increased HFF-1 cell viability by 38.78% under UVA irradiation. At this concentration, COL-Ⅰ expression showed a 77.0-fold increase relative to the control group. In simulated sunlight-induced damage, cerium oxide at 0.039 mg/mL increased COL-Ⅲ expression to 14.06 ng/mL, representing a 1.5-fold increase compared to the negative control group. 【Conclusion】 Cerium oxide demonstrates potent recyclable radical scavenging properties. By upregulating COL-Ⅰ and COL-Ⅲ expression, it significantly mitigates UVA- and simulated sunlight-induced photoaging damage in HFF-1 cells, indicating considerable potential for development as a novel antioxidant and anti-photoaging active ingredient.
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Key words:
- cerium oxide /
- antioxidant /
- anti-photoaging /
- skin ageing /
- cosmetics ingredient
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