低共熔溶剂提取川明参多糖工艺优化与结构表征

李雅欣; 罗敏; 刘元福; 孙全娇; 黄俊铭; 文建霞; 杜文雅; 张良

doi:10.12187/2026.01.005

February 2026

Article Contents

LI Yaxin, LUO Min, LIU Yuanfu, et al. Process optimization and structural characterization of Chuanminshen violaceum polysaccharides extracted by deep eutectic solvents[J]. Journal of Light Industry, 2026, 41(1): 47-58. doi: 10.12187/2026.01.005

Citation: LI Yaxin, LUO Min, LIU Yuanfu, et al. Process optimization and structural characterization of Chuanminshen violaceum polysaccharides extracted by deep eutectic solvents[J]. Journal of Light Industry, 2026, 41(1): 47-58. doi: 10.12187/2026.01.005 shu

Process optimization and structural characterization of Chuanminshen violaceum polysaccharides extracted by deep eutectic solvents

LI Yaxin ¹ ,
LUO Min ² ,
LIU Yuanfu ² ,
SUN Quanjiao ¹ ,
HUANG Junming ¹ ,
WEN Jianxia ¹ ,
DU Wenya ¹ ,
ZHANG Liang ^1,,

1. School of Food and Biological Engineering, Xihua University, Chengdu 610039, China;
2. Sichuan Union (You Lian) Food Co., Ltd., Chengdu 610039, China

Corresponding author: ZHANG Liang, zhang-liang@foxmail.com
Received Date: 2025-11-17
Accepted Date: 2025-06-20

Abstract

【Objective】 This study aims to explore and optimize a green and efficient extraction technology for Chuanminshen violaceum polysaccharide (CVP) and promote its development and utilization in the field of functional foods. 【Methods】 A deep eutectic solvents(DES)-based extraction method for CVP was established and optimized via single-factor experiments and response surface methodology. Subsequently, the extracted CVP was purified by ion-exchange chromatography column and gel filtration chromatography column, respectively. The structure of CVP was characterized by combining ion chromatography, high-performance gel permeation chromatography, infrared spectroscopy, and scanning electron microscopy. 【Results】 The optimal extraction conditions were determined as follows: choline chloride-citric acid DES system (with a molar ratio of 1∶1 and a water content of 30%), liquid-to-solid ratio of 20∶1, extraction temperature of 70 ℃, and extraction time of 120 min. Under these conditions, the extraction rate of CVP was (44.17 ± 0.19)%, an increase of more than 26% over the traditional extraction method; After purification, two CVP fractions (CVP-1-1 and CVP-2-1) with single relative molecular weight and homogeneous structure were obtained. Among them, CVP-1-1 was composed of glucose and galactose, with a total sugar content of (94.87 ± 0.80)% and a relative molecular weight of 9471 Da; CVP-2-1 was composed of glucose, galactose and rhamnose, with a total sugar content of (92.01 ± 1.77)% and a relative molecular weight of 18 159 Da; Both fractions were pyranose ring polysaccharides containing α-glycosidic bonds and β-glycosidic bonds. Their micro-morphologies were related to relative molecular weight and purification process; the purified polysaccharides exhibited a more porous and loose surface, a higher degree of fragmentation, and differed in relative molecular weight, as well as the degree of fragmentation and aggregation. 【Conclusion】 Applying DES to CVP extraction, the extraction rate is significantly increased compared with the traditional extraction method . Moreover, the obtained CVP has a lower relative molecular weight, which may have high antioxidant activity, providing a reference for the development of CVP-based functional foods.
- Chuanminshen violaceum polysaccharide,
- deep eutectic solvent (DES),
- extraction process,
- structural characterization
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Process optimization and structural characterization of Chuanminshen violaceum polysaccharides extracted by deep eutectic solvents

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