微生物降解烟碱的研究进展

曾畅; 刘璐璐; 王泽伟; 朱纯; 徐亚东; 苏二正; 吴蓉

doi:10.12187/2026.01.010

February 2026

Article Contents

ZENG Chang, LIU Lulu, WANG Zewei, et al. Research progress on microbial degradation of nicotine[J]. Journal of Light Industry, 2026, 41(1): 101-110. doi: 10.12187/2026.01.010

Citation: ZENG Chang, LIU Lulu, WANG Zewei, et al. Research progress on microbial degradation of nicotine[J]. Journal of Light Industry, 2026, 41(1): 101-110. doi: 10.12187/2026.01.010 shu

Research progress on microbial degradation of nicotine

ZENG Chang ¹ ,
LIU Lulu ² ,
WANG Zewei ¹ ,
ZHU Chun ¹ ,
XU Yadong ¹ ,
SU Erzheng ^2,, ,
WU Rong ²

1. Nanjing Cigarette Factory, China Tobacco Jiangsu Industrial Co., Ltd., Nanjing 210019;
2. College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037, China

Corresponding author: SU Erzheng, ezhsu@njfu.edu.cn
Received Date: 2025-04-11
Accepted Date: 2025-07-23

Abstract

Nicotine is the primary alkaloid in tobacco and its processing waste, featuring strong environmental persistence and high biotoxicity. Its emission poses potential hazards to the ecological environment and human health, rendering nicotine degradation a critical link in the green development of the tobacco industry and tobacco processing waste treatment. Compared with agricultural regulation measures, physical and chemical methods—which have limitations in energy consumption, cost, and aroma quality, microbial degradation has attracted considerable attention due to its high efficiency, specificity, and environmental benignity. This paper systematically summarizes the physicochemical properties of nicotine, treatment methods, as well as the main microbial groups for nicotine degradation, their degradation characteristics, and metabolic pathways. Genera such as Arthrobacter and Pseudomonas are currently the most extensively studied nicotine-degrading microorganisms, and they primarily convert nicotine into small-molecular-weight metabolites that can enter the tricarboxylic acid cycle via the pyridine pathway, pyrrolidine pathway, and VPP pathway, with key degrading enzymes playing a central role in these metabolic pathways. Additionally, this paper outlines the application prospects of microbial degradation of nicotine in fields including bioremediation of tobacco waste, tobacco processing and manufacturing, and biopharmaceuticals. Future research will address issues such as the poor environmental adaptability and unstable degradation efficiency of strains, and will focus on developing customized strains, constructing synthetic microbial communities, conducting multi-omics analysis, and optimizing metabolic processes to promote the industrialization of microbial nicotine degradation technology.
- microbial degradation,
- nicotine,
- tobacco processing,
- waste governance
References
1. [1]
  付晨青,仝银杏,王风芹,等.我国烟秆资源分布与利用途径[J].纤维素科学与技术,2015,23(2):74-79.
  FU C Q,TONG Y X,WANG F Q,et al.Resources distribution and the utilization way of tobacco stem in China[J].Journal of Cellulose Science and Technology,2015,23(2):74-79.
2. [2]
  LI H J,LI X M,DUAN Y Q,et al.Biotransformation of nicotine by microorganism:The case of Pseudomonas spp[J].Applied Microbiology and Biotechnology,2010,86(1):11-17.
3. [3]
  CIVILINI M,DOMENIS C,SEBASTIANUTTO N,et al.Nicotine decontamination of tobacco agro-industrial waste and its degradation by micro-organisms[J].Waste Management & Research,1997,15(4):349-358.
4. [4]
  CHEN B,SUN L L,ZENG G Y,et al.Gut bacteria alleviate smoking-related NASH by degrading gut nicotine[J].Nature,2022,610(7932):562-568.
5. [5]
  HENDERSON E,RODRIGUEZ GUERRERO L A,CONTINENTE X,et al.Measurement of airborne nicotine,as a marker of secondhand smoke exposure,in homes with residents who smoke in 9 European countries[J].Environmental Research,2023,219:115118.
6. [6]
  SINGHAL A,JAISWAL P K,THAKUR I S.Biopulping of bagasse by Cryptococcus albidus under partially sterilized conditions[J].International Biodeterioration & Biodegradation,2015,97:143-150.
7. [7]
  ZHANG L Y,MAI J,SHI J F,et al.Study on tobacco quality improvement and bacterial community succession during microbial co-fermentation[J].Industrial Crops and Products,2024,208:117889.
8. [8]
  申星,张娟,彭宇,等.一株降解烟碱烟草内生菌的筛选与鉴定[J].农产品加工(学刊),2013(14):69-71. SHEN X,ZHANG J,PENG Y,et al.Screening and identification of one nicotine degrading tobacco entophytes [J].Academic Periodical of Farm Products Processing,2013
  (14):69-71.
9. [9]
  WITTENBERG R E,WOLFMAN S L,DE BIASI M,et al.Nicotinic acetylcholine receptors and nicotine addiction:A brief introduction[J].Neuropharmacology,2020,177:108256.
10. [10]
  AL-DAHHAN W H,KADHOM M,YOUSIF E,et al.Extraction and determination of nicotine in tobacco from selected local cigarettes brands in Iraq[J].Letters in Applied NanoBioScience,2022,11(1):3278-3290.
11. [11]
  龙章德,韦方立,李俊芳,等.一株高浓度烟碱降解菌的筛选、分离和初步鉴定[J].基因组学与应用生物学,2013,32(5):612-615.
  LONG Z D,WEI L F,LI J F,et al.Screening,isolation and identification of a high concentration nicotine degradation bacterium strain[J].Genomics and Applied Biology,2013,32(5):612-615.
12. [12]
  VEROVŠEK T,HEATH D,HEATH E.Occurrence,fate and determination of tobacco (nicotine)and alcohol (ethanol)residues in waste-and environmental waters[J].Trends in Environmental Analytical Chemistry,2022,34:e00164.
13. [13]
  LISUMA J B,MBEGA E R,NDAKIDEMI P A.Nicotine release at the tobacco rhizosphere and their adsorption capacities in different soil textures[J].Rhizosphere,2020,15:100210.
14. [14]
  MAITI M,SARKAR M,LIU D H.Mechanism of nicotine degradation and adsorption by a nano-TiO₂ engineered reduced graphene oxide composite in light variant conditions[J].Catalysis Science & Technology,2020,10(9):2797-2809.
15. [15]
  LISUMA J,MBEGA E,NDAKIDEMI P.Influence of nicotine released in soils to the growth of subsequent maize crop,soil bacteria and Fung[J].Life Sciences and Bio-engineering,2019,22:1-12.
16. [16]
  YANG C Y,YAN B.Dual-function platform based on postsynthetic functionalization of a water-stable hydrogen-bonded organic framework:Ratiometric sensing of nicotine and cotinine and dynamic anticounterfeiting for information encryption[J].Inorganic Chemistry,2023,62(49):20458-20466.
17. [17]
  杨全柳.烤烟新品系YZ15的选育和特性特征研究[D].长沙:湖南农业大学,2023. YANG Q L.Breeding and characterization of a new flue-cured tobacco strain YZ15[D].Changsha:Hunan Agricultural University,2023.
18. [18]
  MOLINA-HIDALGO F J,VAZQUEZ-VILAR M,D’ANDREA L,et al.Engineering metabolism in Nicotiana species:A promising future[J].Trends in Biotechnology,2021,39(9):901-913.
19. [19]
  LEWIS R S,LOPEZ H O,BOWEN S W,et al.Transgenic and mutation-based suppression of a berberine bridge enzyme-like (BBL)gene family reduces alkaloid content in field-grown tobacco[J].PLoS One,2015,10(2):e0117273.
20. [20]
  JAVED M,KASHIF M,ALI A,et al.Assessing impact of manual topping and suckericide application at different stages on FCV tobacco quality and yield [J].Turkish Journal of Agriculture-Food Science and Technology,2024,12(4):596-600.
21. [21]
  LI L,ZHANG H F,WEN J,et al.Direct determination of free nicotine content in tobacco[J].ACS Omega,2022,7(27):23061-23068.
22. [22]
  许志文,王梦雅,谭淞彦,等.降低烟草工业废弃物中烟碱含量的研究[J].天津农业科学,2022,28(5):81-86.
  XU Z W,WANG M Y,TAN S Y,et al.Study on reducing nicotine content in tobacco industry waste [J].Tianjin Agricultural Sciences,2022,28(5):81-86.
23. [23]
  李振杰,王钰田,蒋昆明,等.烟梗丝模板制备TiO₂及其光催化降解尼古丁的研究[J].安全与环境学报,2022,22(5):2791-2798.
  LI Z J,WANG Y T,JIANG K M,et al.Study on preparation of TiO₂ by tobacco stem as a template and its photocatalytic degradation of Nicotine[J].Journal of Safety and Environment,2022,22(5):2791-2798.
24. [24]
  QI C X,CHEN H X,CHEN X F,et al.In-situ-reduced synthesis of cyano group modified g-C₃N₄/CaCO₃ composite with highly enhanced photocatalytic activity for nicotine elimination[J].Journal of Environmental Sciences,2023,126:517-530.
25. [25]
  LI Z J,YANG D D,WEI Z Y,et al.Reduction of nicotine content in tobacco through microbial degradation:Research progress and potential applications[J].Biotechnology for Biofuels and Bioproducts,2024,17(1):144.
26. [26]
  苏丹,林智慧,王雪仁,等.1株高效降烟碱烟草内生菌的筛选、鉴定及其代谢途径研究[J].沈阳农业大学学报,2021,52(3):279-286.
  SU D,LIN Z H,WANG X R,et al.Screening and identification of a highly efficient nicotine-degrading endophyte in tobacco and its metabolic pathways[J].Journal of Shenyang Agricultural University,2021,52(3):279-286.
27. [27]
  WANG H H,YIN B,PENG X X,et al.Biodegradation of nicotine by newly isolated Pseudomonas sp.CS3 and its metabolites[J].Journal of Applied Microbiology,2012,112(2):258-268.
28. [28]
  GONG X W,YANG J K,DUAN Y Q,et al.Isolation and characterization of Rhodococcus sp.Y22 and its potential application to tobacco processing[J].Research in Microbiology,2009,160(3):200-204.
29. [29]
  韩娜娜.烟碱降解细菌的筛选及应用研究[D].济南:齐鲁工业大学,2019. HAN N N.Study on the screening and application of nicotine-degrading bacteria[D].Jinan:Qilu University of Technology,2019.
30. [30]
  TSURUTA T,UMENAI D,HATANO T,et al.Screening micro-organisms for cadmium absorption from aqueous solution and cadmium absorption properties of Arthrobacter nicotianae[J].Bioscience,Biotechnology,and Biochemistry,2014,78(10):1791-1796.
31. [31]
  RATHBONE D A,LISTER D L,BRUCE N C.Biotransformation of alkaloids[J].The Alkaloids Chemistry and Biology,2002,58:1-82.
32. [32]
  夏振远,雷丽萍,吴玉萍,等.降烟碱细菌-烟草节杆菌K9的分离及鉴定[J].中国烟草科学,2006,27(2):1-4.
  XIA Z Y,LEI L P,WU Y P,et al.Isolation and identification of degrading nicotine bacteria-Arthrobacter nicotianae strain K9[J].Chinese Tobacco Science,2006,27(2):1-4.
33. [33]
  RUAN A D,MIN H,PENG X H,et al.Isolation and characterization of Pseudomonas sp.strain HF-1,capable of degrading nicotine[J].Research in Microbiology,2005,156(5/6):700-706.
34. [34]
  XU Z Y,ZHANG T T,HU H Y,et al.Characterization on nicotine degradation and research on heavy metal resistance of a strain Pseudomonas sp. NBB[J].Journal of Hazardous Materials,2023,459:132145.
35. [35]
  ZHAO L,ZHU C J,GAO Y,et al.Nicotine degradation enhancement by Pseudomonas stutzeri ZCJ during aging process of tobacco leaves[J].World Journal of Microbiology and Biotechnology,2012,28(5):2077-2086.
36. [36]
  RAMAN G,MOHAN K,MANOHAR V,et al.Biodegradation of nicotine by a novel nicotine-degrading bacterium,Pseudomonas plecoglossicida TND35 and its new biotransformation intermediates[J].Biodegradation,2014,25(1):95-107.
37. [37]
  YIN R,CHANG M D,MA R,et al.Insights into the imidaclothiz biodegradation by the white-rot fungus Phanerochaete sordida YK-624 under ligninolytic conditions[J].Journal of Agricultural and Food Chemistry,2025,73(21):12877-12886.
38. [38]
  LIU J L,MA G H,CHEN T,et al.Nicotine-degrading microorganisms and their potential applications[J].Applied Microbiology and Biotechnology,2015,99(9):3775-3785.
39. [39]
  MENG X J,LU L L,GU G F,et al.A novel pathway for nicotine degradation by Aspergillus oryzae 112822 isolated from tobacco leaves[J].Research in Microbiology,2010,161(7):626-633.
40. [40]
  WANG Y T,LUO X Y,CHU P,et al.Cultivation and application of nicotine-degrading bacteria and environmental functioning in tobacco planting soil[J].Bioresources and Bioprocessing,2023,10(1):10.
41. [41]
  苏丹.降烟碱内生菌的筛选、鉴定及降解特性研究[D].福州:福建农林大学,2020. SU D.Isolation and identification of nicotine-degrading endophytes and its degradation characteristics[D].Fuzhou:Fujian Agriculture and Forestry University,2020.
42. [42]
  KACHALOVA G S,BOURENKOV G P,MENGESDORF T,et al.Crystal structure analysis of free and substrate-bound 6-hydroxy-l-nicotine oxidase from Arthrobacter nicotinovorans[J].Journal of Molecular Biology,2010,396(3):785-799.
43. [43]
  WANG H X,ZHI X Y,QIU J G,et al.Characterization of a novel nicotine degradation gene cluster ndp in Sphingomonas melonis TY and its evolutionary analysis[J].Frontiers in Microbiology,2017,8:337.
44. [44]
  DENG M Q,BASAK P,ZHANG Y H,et al.An update in recent research on nicotine contamination and nicotine-degrading microorganisms[J].Toxicon,2025,254:108209.
45. [45]
  AHMAD S,CUI D M,ZHONG G H,et al.Microbial technologies employed for biodegradation of neonicotinoids in the agroecosystem[J].Frontiers in Microbiology,2021,12:759439.
46. [46]
  LIAO S,CHENG X X,ZHU L J,et al.Revealing the mechanism of nicotine pyrolysis:Insights from DFT calculations[J].Journal of Analytical and Applied Pyrolysis,2023,176:106245.
47. [47]
  WANG S N,LIU Z,XU P.Biodegradation of nicotine by a newly isolated Agrobacterium sp.strain S33[J].Journal of Applied Microbiology,2009,107(3):838-847.
48. [48]
  RANA S,GUPTA V.Microbial degradation of second generation Neonicotinoid:Thiamethoxam in clay loam soils[J].Journal of Pharmacognosy and Phytochemistry,2019,8(1S):294-298.
49. [49]
  HEGDE D,MANOHARAN T,SRIDAR R.Identification and characterization of bacterial isolates and their role in the degradation of neonicotinoid insecticide thiamethoxam[J].Journal of Pure and Applied Microbiology,2017,11(1):393-400.
50. [50]
  BRIŠKI F,HORGAS N,VUKOVI AC'U1 M,et al.Aerobic composting of tobacco industry solid waste:Simulation of the process[J].Clean Technologies and Environmental Policy,2003,5(3):295-301.
51. [51]
  王瑞,施河丽,陈守文.烟碱降解菌的分离、鉴定及其在烟草秸秆发酵中的应用研究[C]//中国烟草学会.中国烟草学会2015年度优秀论文汇编,2015:1212-1224. WANG R,SHI H L,CHEN S W.Isolation of nicotine degradation bacterium strain and its application in the tobacco straw organic fertilizer composting fermentation[C]//China Tobacco Society.Outstanding Thesis Compilation of China Tobacco Society 2015 Annual,2015
  :1212-1224.
52. [52]
  SHAO Y,ZHONG H,MAO X,et al.Biochar-immobilized Sphingomonas sp.and Acinetobacter sp.isolates to enhance nutrient removal:Potential application in crab aquaculture[J].Aquaculture Environment Interactions,2020,12:251-262.
53. [53]
  郑小嘎,张修国,张天宇,等.真菌菌剂改善烟叶品质的初步研究[J].微生物学通报,2003,30(6):10-13.
  ZHENG X G,ZHANG X G,ZHANG T Y,et al.Improving tobacco quality by fungal leavens[J].Microbiology China,2003,30(6):10-13.
54. [54]
  刘春奎,刘春玲,刘会杰,等.烤烟型卷烟烟气主要化学成分与感官质量的关系[J].南方农业学报,2021,52(6):1665-1673.
  LIU C K,LIU C L,LIU H J,et al.Relationship between main chemical components and sensory quality of flue-cured cigarette[J].Journal of Southern Agriculture,2021,52(6):1665-1673.
55. [55]
  刘春奎,贾琳.烟叶分级[M].北京:中国纺织出版社,2024. LIU C K,JIA L.Tobacco grading[M].Beijing:China Textile Press,2024.
56. [56]
  YANG Y,PENG Q R,OU M Y,et al.Research progress in tobacco fermentation[J].Journal of Biosciences and Medicines,2018,6(6):105-114.
57. [57]
  HRITCU L,MIHASAN M.6-hydroxy-l-nicotine and memory impairment[M]//PREEDY V R.Neuroscience of nicotine:Mechanisms and treatment.Amsterdam: Elsevier,2019:165-172.
58. [58]
  BOIANGIU R S,MIHASAN M,GORGAN D L,et al.Anxiolytic,promnesic,anti-acetylcholinesterase and antioxidant effects of cotinine and 6-hydroxy-L-nicotine in scopolamine-induced zebrafish (Danio rerio)model of Alzheimer’s disease[J].Antioxidants,2021,10(2):212.
59. [59]
  XUE S,SCHLOSBURG J E,JANDA K D.A new strategy for smoking cessation:Characterization of a bacterial enzyme for the degradation of nicotine[J].Journal of the American Chemical Society,2015,137(32):10136-10139.
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Research progress on microbial degradation of nicotine

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