喀什“新新2号”核桃油组成特征及其在HepG2细胞中调节脂质代谢与抗氧化活性评价

郑巧文; 孙怡宁; 邓玮亮; 陈小乐; 陈坤锥; 覃威铭; 申迎宾; 张磊

doi:10.12187/2026.03.004

June 2026

Article Contents

ZHENG Qiaowen, SUN Yining, DENG Weiliang, et al. Compositional characteristics of Kashgar ‘Xinxin 2’ walnut oil and evaluation of its lipid eetabolism-regulating and antioxidant activities in HepG2 cells[J]. Journal of Light Industry, 2026, 41(3): 28-40. doi: 10.12187/2026.03.004

Citation: ZHENG Qiaowen, SUN Yining, DENG Weiliang, et al. Compositional characteristics of Kashgar ‘Xinxin 2’ walnut oil and evaluation of its lipid eetabolism-regulating and antioxidant activities in HepG2 cells[J]. Journal of Light Industry, 2026, 41(3): 28-40. doi: 10.12187/2026.03.004 shu

Compositional characteristics of Kashgar ‘Xinxin 2’ walnut oil and evaluation of its lipid eetabolism-regulating and antioxidant activities in HepG2 cells

1. School of Life Sciences, Guangzhou University, Guangzhou 510006, China;
2. College of Forestry and Landscape Architecture, Xinjiang Agricultural University, Ürümqi 830052, China;
3. College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China

Corresponding author: ZHANG Lei, zlei_xj@sina.com
Received Date: 2025-10-10
Accepted Date: 2025-11-28

Abstract

【Objective】 This study aimed to investigate the chemical composition of Kashgar ‘Xinxin 2’ walnut oil and evaluate its potential to regulate lipid metabolism and exert antioxidant effects. 【Methods】 Chromatographic techniques were used to analyze the fatty acid profile and the contents of minor bioactive components in Kashgar ‘Xinxin 2’ walnut oil. A lipid accumulation model was established in HepG2 cells. Intracellular lipid droplet formation was observed by oil red O staining, and relevant lipid metabolism and oxidative stress markers were determined. 【Results】 The relative content of unsaturated fatty acids in Kashgar ‘Xinxin 2’ walnut oil was 91.01%, with linoleic acid, oleic acid, and α-linolenic acid accounting for 62.70%, 14.30%, and 14.01%, respectively, yielding an ω6/ω3 ratio of 4.48∶1. The oil was also rich in tocopherols (27.06 mg/100 g), phytosterols (332.00 mg/100 g), squalene (86.00 mg/100 g), and polyphenols (6.79 mg/100 g). Treatment with a high dose (500 μg/mL) of the walnut oil significantly inhibited intracellular lipid droplet formation in HepG2 cells, with inhibition rates of 42.19%, 42.21%, 71.96%, and 76.57% for total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), and malondialdehyde (MDA), respectively. Conversely, the levels of high-density lipoprotein cholesterol (HDL-C) and the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) increased to 4.55, 3.15, and 5.10 times those of the model group, respectively. 【Conclusion】 Kashgar ‘Xinxin 2’ walnut oil is rich in unsaturated fatty acids and various minor bioactive components, and it demonstrates significant potential in regulating lipid metabolism and exerting antioxidant effects at the cellular level, suggesting its promise as a novel healthy edible oil capable of modulating lipid metabolism.
- walnut oil,
- compositional characteristic,
- lipid metabolism,
- antioxidant activity,
- HepG2 cells
References
1. [1]
  国家林业和草原局.中国林业和草原统计年鉴2023[M].北京:中国林业出版社,2024:30. National Forestry and Grassland Administration.China forestry and grassland statistical yearbook 2023[M].Beijing:China Forestry Publishing House,2024:30.
2. [2]
  郝金莲,王如月,罗莎莎,等.采收期对叶城县6个核桃品种品质影响初探[J].核农学报,2023,37(3):649-659.
  HAO J L,WANG R Y,LUO S S,et al.Preliminary study on effect of harvesting times on the quality of six walnut varieties in Yecheng County[J].Journal of Nuclear Agricultural Sciences,2023,37(3):649-659.
3. [3]
  郝金莲.采收期对新疆“温185”和“新新2号”核桃果实品质的影响[D].乌鲁木齐:新疆农业大学,2022. HAO J L.Effect of harvesting time on walnut fruit quality of Xin Jiang J.regia ‘Wen 185’and J.regia ‘Xinxin 2’[D].Ü
  ;rümqi:Xinjiang Agricultural University,2022.
4. [4]
  NGUYEN T H D,VU D C.A review on phytochemical composition and potential health-promoting properties of walnuts[J].Food Reviews International,2023,39(1):397-423.
5. [5]
  YAN S X,WANG X S,YANG C K,et al.Insights into walnut lipid metabolism from metabolome and transcriptome analysis[J].Frontiers in Genetics,2021,12:715731.
6. [6]
  GAO P,LIU R J,JIN Q Z,et al.Key chemical composition of walnut (Juglans regia.L) oils generated with different processing methods and their cholesterol-lowering effects in HepG2 cells[J].Food Bioscience,2022,45:101436.
7. [7]
  GU J,SHI Y N,ZHU N,et al.Celastrol functions as an emerging manager of lipid metabolism:Mechanism and therapeutic potential[J].Biomedicine & Pharmacotherapy,2023,164:114981.
8. [8]
  JEEYAVUDEEN M S,KHAN S K A,FOUDA S,et al.Management of metabolic-associated fatty liver disease:The diabetology perspective[J].World Journal of Gastroenterology,2023,29(1):126-143.
9. [9]
  KANG E S,HUR J,JO Y,et al.Comparative effects of nanoemulsions loaded with duck oil and lard oil on palmitate-induced lipotoxicity[J].Journal of Food Biochemistry,2020,44(2):e13117.
10. [10]
  ONTAWONG A,BOONPHANG O,PASACHAN T,et al.Hepatoprotective effect of coffee pulp aqueous extract combined with simvastatin against hepatic steatosis in high-fat diet-induced obese rats[J].Journal of Functional Foods,2019,54:568-577.
11. [11]
  WONGPHUKHIAW S,JAKO P,PUENGPAN S,et al.Beneficial effects of molasses extract in treatment of nonalcoholic fatty liver disease in high-fat diet-induced obese rats through regulation of lipid metabolism,bile acid,and oxidative stress[J].Journal of Agriculture and Food Research,2025,22:102085.
12. [12]
  ZHOU Y J,XU N,ZHANG X C,et al.Chrysin improves glucose and lipid metabolism disorders by regulating the AMPK/PI3K/AKT signaling pathway in insulin-resistant HepG2 cells and HFD/STZ-induced C57BL/6J mice[J].Journal of Agricultural and Food Chemistry,2021,69(20):5618-5627.
13. [13]
  WANG J K,HE Y T,YU D Q,et al.Perilla oil regulates intestinal microbiota and alleviates insulin resistance through the PI3K/AKT signaling pathway in type-2 diabetic KKAy mice[J].Food and Chemical Toxicology,2020,135:110965.
14. [14]
  XIN M L,WANG H L,WANG M,et al.Attenuating effect of Polygala tenuifolia Willd.seed oil on progression of MAFLD[J].Frontiers in Pharmacology,2023,14:1253715.
15. [15]
  中华人民共和国国家卫生和计划生育委员会,国家食品药品监督管理总局.食品安全国家标准食品中脂肪酸的测定:GB 5009.168—2016 [S].北京:中国标准出版社,2017. National Health and Family Planning Commission of The People’s Republic of China,China Food and Drug Administration.National food safety standard—Determination of fatty acid in foods:GB 5009.168—2016
  [S].Beijing:Chinese Specification Press,2017.
16. [16]
  中华人民共和国国家卫生和计划生育委员会,国家食品药品监督管理总局.食品安全国家标准食品中维生素A、D、E的测定:GB 5009.82—2016[S].北京:中国标准出版社,2017. National Health and Family Planning Commission of The People’s Republic of China,China Food and Drug Administration.National food safety standard—Determination of vitamins a,d and e in foods:GB 5009.82—2016
  [S].Beijing:Chinese Specification Press,2017.
17. [17]
  全国粮油标准化技术委员会.动植物油脂甾醇组成和甾醇总量的测定气相色谱法:GB/T 25223—2010[S].北京:中国标准出版社,2010. National Grain and Oil Standardization Technical Committee.Animal and vegetable fats and oils—Determination of individual and total sterols contents—Gas chromatographic method:GB/T 25223—2010[S].Beijing:Chinese Specification Press,2010.
18. [18]
  国家市场监督管理总局,国家标准化管理委员会.动植物中角鲨烯含量的测定:GB/T 43732—2024[S].北京:中国标准出版社,2024. National Biochemical Testing STandardization Technical Committee (SAC/TC 387).Determination of squalene in animals and vegetables:GB/T 43732—2024
  [S].Beijing:Chinese Specification Press,2024.
19. [19]
  全国粮油标准化技术委员会(SAC/TC 270).粮油检验植物油中多酚的测定分光光度法:LS/T 6119—2017[S].北京:中国标准出版社,2017. National Grain and Oil Standardization Technical Committee (SAC/TC 270).Inspection of grain and oils—Determination of polyphenols in vegetable oil—Spectrometric method:LS/T 6119—2017
  [S].Beijing:Chinese Specification Press,2017.
20. [20]
  中华人民共和国国家卫生健康委员会,国家市场监督管理总局.食品安全国家标准食品中多环芳烃的测定:GB 5009.265—2021[S].北京:中国标准出版社,2021. National Health Commission of The People’s Republic of China,State Administration for Market Regulation.National food safety standard—Determination of polycyclic aromatic hydrocarbons in foods:GB 5009.265—2021
  [S].Beijing:Chinese Specification Press,2021.
21. [21]
  BALAMURUGAN V T,HARITHA J,ARUNCHENDHURAN R,et al.Classification of groundnut oil using advanced ATR-MIR spectroscopy and chemometrics[J].Food Analytical Methods,2022,15(7):1778-1786.
22. [22]
  LIAO J Q,NAI Y F,FENG L,et al.Walnut oil prevents scopolamine-induced memory dysfunction in a mouse model[J].Molecules,2020,25(7):1630.
23. [23]
  高盼.我国核桃油的组成特征及其抗氧化和降胆固醇功效评估[D].无锡:江南大学,2019. GAO P.Chemical composition antioxidant capacity and cholesterol-lowering effect of walnut oil in China[D].Wuxi:Jiangnan University,2019.
24. [24]
  CHEN X,RAN J Q,MAZHAR M,et al.The balanced unsaturated fatty acid supplement constituted by woody edible oils improved lipid metabolism and gut microbiota in high-fat diet mice[J].Frontiers in Nutrition,2023,10:1203932.
25. [25]
  YANG H B,XIAO X,LI J J,et al.Chemical compositions of walnut (Juglans spp.)oil:Combined effects of genetic and climatic factors[J].Forests,2022,13(6):962.
26. [26]
  RABADÁN A,PARDO J E,PARDO-GIMÉNEZ A,et al.Effect of genotype and crop year on the nutritional value of walnut virgin oil and defatted flour[J].Science of the Total Environment,2018,634:1092-1099.
27. [27]
  PYCIA K,KAPUSTA I,JAWORSKA G,et al.Antioxidant properties,profile of polyphenolic compounds and tocopherol content in various walnut (Juglans regia L.)varieties[J].European Food Research and Technology,2019,245(3):607-616.
28. [28]
  MITSIKARIS P D,KOKOKIRIS L,PRITSA A,et al.Investigating the tocopherol contents of walnut seed oils produced in different European countries analyzed by HPLC-UV:A comparative study on the basis of geographical origin[J].Foods,2022,11(22):3719.
29. [29]
  王蒙蒙,寇宇星,周笙,等.不饱和脂肪酸室温氧化过程中自由基的变化[J].食品科学,2021,42(11):56-62.
  WANG M M,KOU Y X,ZHOU S,et al.Changes of free radicals during oxidation of unsaturated fatty acids at room temperature[J].Food Science,2021,42(11):56-62.
30. [30]
  RUSU M E,FIZESAN I,POP A,et al.Walnut (Juglans regia L.)Septum:Assessment of bioactive molecules and in vitro biological effects[J].Molecules,2020,25(9):2187.
31. [31]
  DURMAZ G,GOKMEN V.Effect of refining on bioactive composition and oxidative stability of hazelnut oil[J].Food Research International,2019,116:586-591.
32. [32]
  LIU L F,CAI H L,ZHANG Y F,et al.Chemical compositions and oxidative stabilities of cold-pressed walnut oils (Juglans regia L.):Effects of chemical refining,water degumming,and molecular distillation[J].Journal of Food Science,2024,89(11):7589-7598.
33. [33]
  SONG H Y,CONG Z F,WANG C L,et al.Research progress on Walnut oil:Bioactive compounds,health benefits,extraction methods,and medicinal uses[J].Journal of Food Biochemistry,2022,46(12):e14504.
34. [34]
  RÉBUFA C,ARTAUD J,LE DRÉAU Y.Walnut (Juglans regia L.)oil chemical composition depending on variety,locality,extraction process and storage conditions:A comprehensive review[J].Journal of Food Composition and Analysis,2022,110:104534.
35. [35]
  RABADÁN A,ÁLVAREZ-ORTÍ M,PARDO J E.A comparison of the effect of genotype and weather conditions on the nutritional composition of most important commercial nuts[J].Scientia Horticulturae,2019,244:218-224.
36. [36]
  MA X,HUANG C B,ZHENG C,et al.Effect of oil extraction methods on walnut oil quality characteristics and the functional properties of walnut protein isolate[J].Food Chemistry,2024,438:138052.
37. [37]
  SHEN Y B,ZHENG L Y,PENG Y,et al.Physicochemical,antioxidant and anticancer characteristics of seed oil from three Chenopodium quinoa genotypes[J].Molecules,2022,27(8):2453.
38. [38]
  GAO P,LIU R J,JIN Q Z,et al.Comparison of different processing methods of iron walnut oils (Juglans sigillata):Lipid yield,lipid compositions,minor components,and antioxidant capacity[J].European Journal of Lipid Science and Technology,2018,120(9):1800151.
39. [39]
  HASHIM M,AHMAD B,DROUET S,et al.Comparative effects of different light sources on the production of key secondary metabolites in plants in vitro cultures[J].Plants,2021,10(8):1521.
40. [40]
  WEI J C,YANG Y,PENG Y,et al.Biosynthesis and the transcriptional regulation of terpenoids in tea plants (Camellia sinensis)[J].International Journal of Molecular Sciences,2023,24(8):6937.
41. [41]
  JIAO J,XU X J,LU Y,et al.Identification of genes associated with biosynthesis of bioactive flavonoids and taxoids in Taxus cuspidata Sieb.et Zucc.plantlets exposed to UV-B radiation[J].Gene,2022,823:146384.
42. [42]
  LI A R,DU Q H,ZENG Y L,et al.Light regulated CoWRKY15 acts on CoSQS promoter to promote squalene synthesis in Camellia oleifera seeds[J].International Journal of Molecular Sciences,2024,25(20):11134.
43. [43]
  LI Y Q,KONG D X,FU Y,et al.The effect of developmental and environmental factors on secondary metabolites in medicinal plants[J].Plant Physiology and Biochemistry,2020,148:80-89.
44. [44]
  GU Y Y,ZHU Z Y,ZENG Y L,et al.CoHMGR2 as a critical regulator of squalene biosynthesis key period in Camellia oleifera seed kernels[J].Physiologia Plantarum,2025,177(3):e70352.
45. [45]
  GAO P,CAO Y,LIU R J,et al.Phytochemical content,minor-constituent compositions,and antioxidant capacity of screw-pressed walnut oil obtained from roasted kernels[J].European Journal of Lipid Science and Technology,2019,121(1):1800292.
46. [46]
  ZHANG J J,GAO Y,XU X,et al.In situ rapid analysis of squalene,tocopherols,and sterols in walnut oils based on supercritical fluid chromatography-quadrupole time-of-flight mass spectrometry[J].Journal of Agricultural and Food Chemistry,2023,71(43):16371-16380.
47. [47]
  PYCIA K,KAPUSTA I,JAWORSKA G.Impact of the degree of maturity of walnuts (Juglans regia L.)and their variety on the antioxidant potential and the content of tocopherols and polyphenols[J].Molecules,2019,24(16):2936.
48. [48]
  GRAJZER M,SZMALCEL K,KUZ'MIN'SKI Ł,et al.Characteristics and antioxidant potential of cold-pressed oils:Possible strategies to improve oil stability[J].Foods,2020,9(11):1630.
49. [49]
  SADIGHARA P.Antioxidants as modulators of PAH contaminants in food and living organisms:An overview study[J].Polycyclic Aromatic Compounds,2025,45(1):123-135.
50. [50]
  WANG J W,ZHANG K,SHENG J,et al.Investigation on walnut kernel oil extraction using different methods[J].BioResources,2023,18(1):869-883.
51. [51]
  刘畅.东北山核桃油绿色提取及其体外活性评价[D].哈尔滨:东北林业大学,2022. LIU C.Green extraction of Manchurian walnuts kernel oil and its preliminary evaluation of in vitro biological activities[D].Harbin:Northeast Forestry University,2022.
52. [52]
  帅希祥.澳洲坚果油组成、营养及其油凝胶体系构建和应用[D].南昌:南昌大学,2023. SHUAI X X.Composition and nutrition of macadamia oil and construction of oleogel system and its application[D].Nanchang:Nanchang University,2023.
53. [53]
  ZHANG Y D,DAI Z Z,PU S M,et al.Mechanistic studies on the alleviation of lipid accumulation and oxidative stress by Majia pomelo seed oil[J].Food Bioscience,2024,62:105322.
54. [54]
  KING T W,COCHRAN B J,RYE K A.ApoA-I and diabetes[J].Arteriosclerosis,Thrombosis,and Vascular Biology,2023,43(8):1362-1368.
55. [55]
  ZANOTTI I,POT F,CUCHEL M.HDL and reverse cholesterol transport in humans and animals:Lessons from pre-clinical models and clinical studies[J].Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids,2022,1867(1):159065.
56. [56]
  ZHANG Y,WU L Y,YAO Z X,et al.Hypolipidemic effects of hickory nut oil using cold pressure extraction[J].Food Science and Biotechnology,2016,25(1):41-46.
57. [57]
  SHEN Y B,ZHENG L Y,JIN J,et al.Phytochemical and biological characteristics of Mexican Chia seed oil[J].Molecules,2018,23(12):3219.
58. [58]
  SU J H,MA C Y,LIU C X,et al.Hypolipidemic activity of peony seed oil rich in α-linolenic,is mediated through inhibition of lipogenesis and upregulation of fatty acid β-oxidation[J].Journal of Food Science,2016,81(4):H1001-H1009.
59. [59]
  ZHU H Y,CHEN L J,CHEN Z X,et al.Synthesis of cholesterol analogues and comparison on their effect on plasma cholesterol with β-Sitosterol[J].Food Chemistry,2024,461:140820.
60. [60]
  XU L,LI W,CHEN S Y,et al.Oenothein B ameliorates hepatic injury in alcoholic liver disease mice by improving oxidative stress and inflammation and modulating the gut microbiota[J].Frontiers in Nutrition,2022,9:1053718.
61. [61]
  CLARE K,DILLON J F,BRENNAN P N.Reactive oxygen species and oxidative stress in the pathogenesis of MAFLD[J].Journal of Clinical and Translational Hepatology,2022,10(5):939-946.
62. [62]
  GAO Y,LI C,CHEN B,et al.Anti-hyperlipidemia and antioxidant activities of Amygdalus pedunculata seed oil[J].Food & Function,2016,7(12):5018-5024.
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沈阳化工大学材料科学与工程学院沈阳 110142

Compositional characteristics of Kashgar ‘Xinxin 2’ walnut oil and evaluation of its lipid eetabolism-regulating and antioxidant activities in HepG2 cells

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