SUN Yuan-lin, SHAN Fang, BIAN Jun-sheng and et al. Preparation and analysis of alkali-soluble polysaccharide from tartary buckwheat bran[J]. Journal of Light Industry, 2011, 26(2): 1-4. doi: 10.3969/j.issn.1004-1478.2011.02.001
Citation:
SUN Yuan-lin, SHAN Fang, BIAN Jun-sheng and et al. Preparation and analysis of alkali-soluble polysaccharide from tartary buckwheat bran[J]. Journal of Light Industry, 2011, 26(2): 1-4.
doi:
10.3969/j.issn.1004-1478.2011.02.001
Preparation and analysis of alkali-soluble polysaccharide from tartary buckwheat bran
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Dept.of Life Sci., Yuncheng Univ., Yuncheng 044000, China;
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Inst.of Farm Products Comprehensive Utilization, Shanxi Academy of Agr.Sci., Taiyuan 030031, China
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Received Date:
2010-12-28
Available Online:
2011-03-15
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Abstract
With tartary buckwheat bran as material,alkali-soluble polysaccharide was extracted with 0.2 mol/L NaOH solution and purified on DEAE-Sepharose CL-6B chromatography.The results showed that ABP was mainly composed of glucose,along with small amount of arabinose,xylose,and mannose,which was mainly β-gulcan.ABP was fractionated into 2 fractions on DEAE-Sepharose CL-6B column:ABP1 and ABP2.ABP1,with 85.30% of polysaccharide,consisted mainly of glucose,and small amount of xylose.The molar ratio was n(Glc):n(Xyl)=9.47:1.The molecular weight was 8.5×104 determined by high-performance size-exclusion chromatography.
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References
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Proportional views
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