柽柳核纤孔菌分子鉴定及基于ITS序列的系统发育分析

魏涛; 何培新; 刘伟; 耿卢婧

doi:10.3969/j.issn.1004-1478.2011.04.005

July 2011

Article Contents

WEI Tao, HE Pei-xin, LIU Wei and et al. Molecular characterization of lnocutis tamaricis and phylogenetic analysis of related taxa using ITS sequence analysis[J]. Journal of Light Industry, 2011, 26(4): 22-26. doi: 10.3969/j.issn.1004-1478.2011.04.005

Citation: WEI Tao, HE Pei-xin, LIU Wei and et al. Molecular characterization of lnocutis tamaricis and phylogenetic analysis of related taxa using ITS sequence analysis[J]. Journal of Light Industry, 2011, 26(4): 22-26. doi: 10.3969/j.issn.1004-1478.2011.04.005 shu

Molecular characterization of lnocutis tamaricis and phylogenetic analysis of related taxa using ITS sequence analysis

College of Food and Bioeng., Zhengzhou Univ. of Light Ind., Zhengzhou 450002, China

Received Date: 2011-06-06
Available Online: 2011-07-15

Abstract

Inocutis tamaricis 6-27,collected in Zhengzhou of He'nan province,China,was identified primarily based on morphological characteristics.The rDNA ITS region of the strain was cloned and sequenced,and BLAST matched with GenBank database on homology.The ITS sequences,together with 22 reference sequences obtained from the GenBank database,were used to construct a phylogenetic tree.The results demonstrated that the ITS sequence analysis supported the identification based on morphological characteristics,the strain was Inocutis tamaricis.In phylogenetic analysis,twenty three materials from different genera were divided into six groups.Group A included seven strains of I.tamaricis and one strain of Inonotus cuticularis.I.jamaicensis 4508 from southern hemisphere from Argentina and Fomitiporella caryophylli CBS 448.76 from India were integrated into group B,as big sister group with group A and small sister group with group C,which included two Fulvifomes species.The remained nine strains of Inonotus,Phellinus,Mensularia and Fuscoporia were integrated into group D,E and F.The phylogenetic analysis of allied taxa suggested the independent genus level of Inocutis and Fulvifomes,however,the heterogeneous status of genera Inonotus and Phellinus needs further standardizing and unifying.
- ITS sequence analysis,
- classification and identification,
- phylogeny,
- Hymenochaetales,
- macrofungi
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