ZHANG Fei, WEI Tao, LIU Yin and et al. Construction and expression of prokaryotic vector of valine-pyruvate transaminase gene[J]. Journal of Light Industry, 2013, 28(1): 7-10. doi: 10.3969/j.issn.2095-476X.2013.01.002
Citation:
ZHANG Fei, WEI Tao, LIU Yin and et al. Construction and expression of prokaryotic vector of valine-pyruvate transaminase gene[J]. Journal of Light Industry, 2013, 28(1): 7-10.
doi:
10.3969/j.issn.2095-476X.2013.01.002
Construction and expression of prokaryotic vector of valine-pyruvate transaminase gene
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Received Date:
2012-08-23
Available Online:
2013-01-15
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Abstract
The engineered strain of Escherichia coli with valine-pyruvate transaminase gene was constructed and the expression condition for valine-pyruvate transaminase was optimized.The result of PCR showed that a special DNA band was amplified and the length of the band was accord with the length of avtA,1 254 bp.Activity of valine-pyruvate transaminase was found by paper chromatography but the enzyme activity was not high.The expression of valine-pyruvate transaminase was evaluated by SDS-PAGE and a high expression was displayed.The optimal conditions were peptone 12 g/L,IPTG 0.4 mmol/L and induced time 8 h.
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Proportional views
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