JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

Volume 29 Issue 3
May 2014
Article Contents
ZHAO Xue-wei, JIA Tao, ZHANG Xue-lin, et al. FT-IR spectroscopic characterization of soybean protein isolates after thermo-mechanical treatments[J]. Journal of Light Industry, 2014, 29(3): 12-16. doi: 10.3969/j.issn.2095-476X.2014.03.003
Citation: ZHAO Xue-wei, JIA Tao, ZHANG Xue-lin, et al. FT-IR spectroscopic characterization of soybean protein isolates after thermo-mechanical treatments[J]. Journal of Light Industry, 2014, 29(3): 12-16. doi: 10.3969/j.issn.2095-476X.2014.03.003 shu

FT-IR spectroscopic characterization of soybean protein isolates after thermo-mechanical treatments

  • Received Date: 2014-03-19
    Available Online: 2014-05-15
  • Soybean protein isolate(SPI) with a moisture content of 60% was thermo-mechanically treated torque rheometer at 30℃ and 90℃ for 2 min,15 min and 30 min respectively,and characterized by Fourier transform infrared (FT-IR) spectroscopy.The results showed that after thermo-mechanical treatments,the symmetrical and asymmetrical stretching of C-H bonds in -CH2 resides became weak and even disappeared the stretching vibration and asymmetrical stretching of C-H bonds drifted to higher position.The α-helix and β-turn decreased accompanying with β-sheet and random coil increased.For that lower moisture content SPI system,the mechanical effect during thermo-mechanical processing may be the major contributor to the transformation of secondary protein structure.
  • 加载中
    1. [1]

      Wellner N,Belton P S,Tatham A S.Fourier transform IR spectroscopic study of hydration-induced structure changes in the solid state of x-gliadins[J].Biochem J,1996,319:741.

    2. [2]

      刘燕燕,曾新安,陈晓东.FTIR分析脉冲电场和热处理后的大豆分离蛋白结构变化[J].光谱学与光谱分析,2010, 30(9):2340.

    3. [3]

      Tang C H,Ma C Y.Effect of high pressure treatment on aggregation and structuralproperties of soy protein isolate[J].LWT-Food Science and Technology,2009,42:606.

    4. [4]

      Muriel S,Isbelle K,Jacques C.Molecular basis of film formation from a soybean protein:comparison between the conformation of glycinin in aqueous solution and in films[J].Int J Biol Macromol,1998,23:241.

    5. [5]

      Chen X,Ru Y,Chen F,et al.FTIR spectroscopic characterization of soy proteins obtained through AOT reverse micelles[J].Food Hydrocol,2013,31:435.

    6. [6]

      Gonzalez A,Strumia M C,Igarzabal C I A.Cross-linked soy protein as material for biodegradable films:synthesis,characterization and biodegradation[J].J Food Eng,2011,106:331.

    7. [7]

      Kealley C S,Rout M K,Dezfouli M R.Structure and molecular mobility of soy glycinin in the solid state[J].Biomacromol,2008,9(10):2937.

    8. [8]

      张彩猛,华欲飞,孔祥珍.高速剪切对醇法大豆浓缩蛋白溶解特征的影响[J].大豆科学,2010,29:853.

    9. [9]

      Surewicz W K,Mantsch H H.New insight into protein secondary structure from resolution-enhanced infrared spectra[J].Biochimica et Biophysica Acta,1988,952(2):115.

    10. [10]

      Steinbach P J,Brooks B R.Protein hydration elucidated by molecular dynamics simulation[J].Proc Natl Acad Sci USA,1993,90:9135.

    11. [11]

      Schmidt V,Giacomelli C,Soldi V.Thermal stability of films formed by soy protein isolateesodium dodecyl sulfate[J].Polym Degrad Stab,2005,87:25.

    12. [12]

      Li C E,Chalmers J M,Griffiths P R.Applications of Vibrational Spectroscopy in Food[M].New Jersey:Wiley,2010.

    13. [13]

      Guerrero P,de la Caba K.Thermal and mechanical properties of soy protein films processed at different pH by compression[J].J Food Eng,2010,100:261.

    14. [14]

      Liu Y Y,Zeng X A,Deng Z,et al.Effect of pulsed electric field on the secondary structure and thermal properties of soy protein isolate[J].Eur Food Res Technol,2011,233:841.

    15. [15]

      Pelton J T and McLean L R.Spectroscopic methods for analysis of protein secondary structure[J].Analytical Biochem,2000,277:167.

    16. [16]

      康立宁.大豆蛋白高水分挤压组织化技术和机理研究[D].杨凌:西北农林科技大学,2007

Article Metrics

Article views(914) PDF downloads(43) Cited by()

Ralated
    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return