JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

Volume 29 Issue 4
July 2014
Article Contents
WANG Ming-hua, YANG Guang, ZHANG Yuan-chang, et al. DNA immobilization and hybridization on graphene/gold nanoparticles/polypyrrole nanocomposite[J]. Journal of Light Industry, 2014, 29(4): 6-11. doi: 10.3969/j.issn.2095-476X.2014.04.002
Citation: WANG Ming-hua, YANG Guang, ZHANG Yuan-chang, et al. DNA immobilization and hybridization on graphene/gold nanoparticles/polypyrrole nanocomposite[J]. Journal of Light Industry, 2014, 29(4): 6-11. doi: 10.3969/j.issn.2095-476X.2014.04.002 shu

DNA immobilization and hybridization on graphene/gold nanoparticles/polypyrrole nanocomposite

  • Corresponding author: ZHANG Zhi-hong, 
  • Received Date: 2014-06-13
    Available Online: 2014-07-15
  • Graphene/gold nanoparticles/polypyrrole (G/AuNPs/PPy) nanocomposite was prepared by in-situ chemical oxidation method,then DNA was immobilized and hybridized on the surface of the composite.The chemical structure,element composition and surface morphology of the nanocomposite were characterized by Fourier transform infrared spectroscopy,X-ray photoelectron spectroscopy,field emission scanning electron microscope and transmission electron microscopy,respectively.Afterwards,electrochemical cyclic voltammetry,impedance spectroscopy and electrochemical quartz crystal microbalance were used to determine the variation of electrochemical properties and weight of the nanocomposite before and after DNA immobilization/hybridization.It demonstrated that the electrochemical activities of G/AuNPs/PPy were better than that of G/AuNPs nanocomposite.The immobilization amount of DNA on the G/AuNPs/PPy nanocomposite was 307 ng,and aslo 357 ng of perfectly matched target DNA was detected on the surface.
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    1. [1]

      Kuila T,Bose S,Khanra P,et al.Recent advances in gra-phene-based biosensors[J].Biosen Bioelectron,2011,26(12):4637.

    2. [2]

      李聪聪,孙向英,杨传孝.基于石墨烯和量子点自组装膜的能量转移构筑的高灵敏度DNA界面荧光传感[J].高等学校化学学报,2012,33(7):1367.

    3. [3]

      张治红,时宇,刘顺利,等.氧化亚铜/石墨烯纳米复合材料的制备及性能研究[J].郑州轻工业学院学报:自然科学版,2013,28(2):44.

    4. [4]

      Xia H B,Narayanan J,Cheng D M,et al.Formation of ordered arrays of oriented polyaniline nanoparticle nanorods[J].J Phys Chem B,2005,109(26):12677.

    5. [5]

      Li Y,Zhang W X,Chang J,et al."Click" on conducting polymer coated electrodes:A versatile platform for the modification of electrode surfaces[J].Macromol Chem Phys,2008,209(3):322.

    6. [6]

      Dong H,Cao X D,Li C M,et al.An in situ electrochemical surfaceplasmon resonance immunosensor with polypyrrole propylic acid film:Comparison between SPR and electrochemical responses from polymer formation to protein immunosensing[J].Biosens Bioelectron,2008,23(7):1055.

    7. [7]

      Davies A,Audette P,Farrow B,et al.Graphene-basedflexible supercapacitors:Pulse-electropolymerization of polypyrrole on free-standing graphene films[J].J Phys Chem C,2011,115(35):17612.

    8. [8]

      刘建华,马玉骁,于美,等.石墨烯-聚吡咯纳米管杂化材料的制备及电容特性[J].无机化学学报,2013,29(5):929.

    9. [9]

      Han X,Fang X,Shi A,et al.An electrochemical DNAbiosensor based on gold nanorods decorated graphene oxide sheets for sensing platform[J].Anal Biochem,2013,443(2):117.

    10. [10]

      Inagaki M,Kim Y A,Endo M.Graphene:preparation and structural perfection[J].J Mater Chem,2011,21(10):3280.

    11. [11]

      Zhang Z H,Dou J,Yan F F,et al.Plasma polymerized pyrrole films for biological applications:Correlation between protein adsorption properties and characteristics[J].Plasma Process Polym,2011,8(10):923.

    12. [12]

      Sauerbrey G Z.Verwendung von schwingquartzen zur wagung dunner schichten and zur microwagung[J].Z Phys,1959,155(2):206.

    13. [13]

      Bruckenstein S,Swathirajan S.Potential dependence of leadand silver underpotential coverages in acetonitrile using a piezoelectric crystal oscillator method[J].Electrochim.Acta,1985,30(7):851.

    14. [14]

      Wei H,Wang Z D,Yang L M,et al.Lysozyme-stabilized gold fluorescent cluster:Synthesis and application as Hg2+ sensor[J].Analyst,2010,135:1406.

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