JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

Volume 29 Issue 4
July 2014
Article Contents
DENG Mei-zhong, CHEN Du-jian, HUANG Sheng-han and et al. Determination of 3,4,5-trihydroxy benzoic acid in tobacco essence and flavor[J]. Journal of Light Industry, 2014, 29(4): 39-42. doi: 10.3969/j.issn.2095-476X.2014.04.009
Citation: DENG Mei-zhong, CHEN Du-jian, HUANG Sheng-han and et al. Determination of 3,4,5-trihydroxy benzoic acid in tobacco essence and flavor[J]. Journal of Light Industry, 2014, 29(4): 39-42. doi: 10.3969/j.issn.2095-476X.2014.04.009 shu

Determination of 3,4,5-trihydroxy benzoic acid in tobacco essence and flavor

  • Received Date: 2014-03-21
    Available Online: 2014-07-15
  • A method for the detection of 3,4,5-trihydroxy benzoic acid in tobacco essence and flavor was developed by using high performance liquid chromatography.After oscillate-extraction by ethanol,the samples were separated by a C18 column (100 mm×3.9 mm,5 μm),using methanol and 0.05% phosphoric acid (6:94) as mobile phases by isocratic elution,and detected by diode array detector with the wavelength of 272 nm.The experimental results showed that:within the range of 0.1~10.0 mg/L,the linear correlation coefficient for 3,4,5-trihydroxy benzoic acid was 0.999 4,the low detection limit was 1.01 μg/g,the recovery was 93.1%~103.2%,the RSD < 5% (n=5).The method was sensitive,accurate and easy-controlled,and it was suitable for quantitive determination of 3,4,5-trihydroxy benzoic acid in different types of tobacco essence and flavor.
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