Study on biosynthesis of aloe-emodin

Recombinant glucosidase was used to catalysis biosynthesize of aloe-emodin through aloin. Single factor experiment and uniform design were used to optimize the biosynthesize conditions. As the result,the optimal conditons of aleo-emodin's biosynthesis were as following: reaction time 4 h,combinant glucosidase concentration 50 U/mL,the ratio of tert-butanol and water 5:5(v/v),reaction temperature 43℃,and pH value 5.2. Under those conditions,the highest yield was 92.18%.