高效液相色谱法同时测定果蔬汁饮料中20种禁限用食品添加剂

刘胜男; 张国庆; 王素方; 郭保宝; 权明珠; 石志格

doi:10.3969/j.issn.2096-1553.2016.4.003

July 2016

Article Contents

LIU Sheng-nan, ZHANG Guo-qing, WANG Su-fang, et al. Simultaneous determination of 20 restricted food additives in fruit and vegetable juice beverage by HPLC[J]. Journal of Light Industry, 2016, 31(4): 23-29. doi: 10.3969/j.issn.2096-1553.2016.4.003

Citation: LIU Sheng-nan, ZHANG Guo-qing, WANG Su-fang, et al. Simultaneous determination of 20 restricted food additives in fruit and vegetable juice beverage by HPLC[J]. Journal of Light Industry, 2016, 31(4): 23-29. doi: 10.3969/j.issn.2096-1553.2016.4.003 shu

Simultaneous determination of 20 restricted food additives in fruit and vegetable juice beverage by HPLC

Sanmenxia Entry-exit Inspection and Quarantine Bureau of P. R. China, Sanmenxia 472000, China;
Inspection & Quarantine Technology Centre, He'nan Entry-exit Inspection and Quarantine Bureau of P. R. China, Zhengzhou 450003, China

Corresponding author: WANG Su-fang,
Received Date: 2016-04-13
Available Online: 2016-07-15

Abstract

A high performance liquid chromatography (HPLC) method was developed for simultaneous determination of 20 restricted food additives in fruit and vegetable juice beverage.The method was based on extraction with methanol mixed with water.The separation was achieved on a ZORBAX SB-C18 column by using methanol and NH4Ac as mobile phase with the gradient eluent.Components were detected with a diode array detector (DAD).The quantification was performed by the external standard method.The results showed that the method exhibited an excellent linearity in the concentration range of 0.5~50.0 μg/mL with a correlation coefficient greater than 0.999 0 and a good repeatability with relative standard deviation of 1.5%~4.9%.The limits of detection were between 0.5 mg/kg and 12.5 mg/kg,the limit of quantitation were between 2.5 mg/kg and 40.0 mg/kg,and average recoveries ranged from 80.1% to 100.9%.The test result of the additives content of ten types of fruit and vegetable juice beverage on the market showed that the present method was simple,and had good separation effect.Its recovery and accuracy could meet the requirements for the simultaneous determination additives in fruit and vegetable juice beverage.
- high performance liquid chromatography (HPLC),
- food additives,
- fruit and vegetable juice beverage
References
1. [1]
  薛虎寅,尹永梅,张太昌,等.偶氮类合成色素检测技术的研究进展[J].生物技术进展,2012,2(3):171.
2. [2]
  AMIN K,HAMEID H,ABD E A.Effect of food azo dyes tartrazine and carmoisine on biochemical parameters related to renal,hepatic function and oxidative stress biomarkers in young male rats[J].Food Chem Toxicol,2010,48:2994.
3. [3]
  NEERA M V,MEENAL M,RICHA K J.Effect of sunset yellow on testis in rats[J].Ecophysiol Health,2005(5):1.
4. [4]
  汪辉,曹小彦,李林,等.反相高效液相色谱法对果蔬汁饮料中14种常见食品添加剂的快速测定[J].分析测试学报,2009,28(10):1194.
5. [5]
  咸瑞卿,王小兵,李启艳,等.固相萃取-高效液相色谱-二极管阵列检测法同时测定饮料中10种合成着色剂[J].现代食品科技,2013,29(6):1413.
6. [6]
  陈沛金,张毅,肖锋,等.高效液相色谱法测定糖果、蜜饯和饮料中19种食品添加剂[J].食品安全质量检测学报,2014,5(11):3487.
7. [7]
  王宏亮,陈盼盼,何计龙,等.高效液相配备DAD同时鉴别并测定饮料中的甜味剂、防腐剂和色素[J].食品工业,2015,36(7):285.
8. [8]
  吕梦莎,林能镖,梁玉英,等.高效液相色谱法同时测定葡萄酒中16种添加剂[J].食品科学,2016,37(08):222.
9. [9]
  王国庆,弓丽华,王素方,等.基于改进标准加入法的碳酸饮料中多种色素含量的同时测定[J].河南师范大学学报(自然科学版),2014,42(1):67.
10. [10]
  王素方,刘云,弓丽华,等.基于改进标准加入法的碳酸饮料中多种防腐剂和甜味剂高通量筛查分析[J].光谱学与光谱分析,2016,36(2):482.
11. [11]
  吴彦蕾,林晓洋,胡靖,等.高效液相色谱法快速检测食品中18种合成色素[J].食品工业科技,2013,34(24):49.
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Simultaneous determination of 20 restricted food additives in fruit and vegetable juice beverage by HPLC

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