JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

Volume 32 Issue 4
July 2017
Article Contents
QIAN Cun, WANG Yun-long, MING Liang, et al. Study on quantitative detection of tPSA and fPSA by fluorescent chromatography[J]. Journal of Light Industry, 2017, 32(4): 37-42. doi: 10.3969/j.issn.2096-1553.2017.4.006
Citation: QIAN Cun, WANG Yun-long, MING Liang, et al. Study on quantitative detection of tPSA and fPSA by fluorescent chromatography[J]. Journal of Light Industry, 2017, 32(4): 37-42. doi: 10.3969/j.issn.2096-1553.2017.4.006 shu

Study on quantitative detection of tPSA and fPSA by fluorescent chromatography

  • Corresponding author: WANG Yun-long, 
  • Received Date: 2017-03-22
    Available Online: 2017-07-15
  • Conjugating the EDC activated fluorescent microspheres with antibody to prepare the fluorescence chromatographic strip for simultaneous detection of tPSA and fPSA. The performance analysis and methodological comparison for the test strip were further performed. The results showed that the sensitivity for PSA and fPSA detection by the test strip were 0.08 ng/mL and 0.06 ng/mL, respectively. The coefficient of variation within and between groups was less than 10% and 15%, respectively. The recovery rates ranged from 95% to 105%. The specificity of the test strip was well. The results revealed a good correlation between self-prepared test strip and electrochemiluminescence detection, which could satisfy the needs of aided diagnosis of small-scale hospitals.
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