JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

Volume 32 Issue 6
November 2017
Article Contents
HE Pei-xin, LI Cong-cong, HU Xiao-long, et al. Component analysis of volatile metabolites of culturable Clostridium spp. isolated from pit muds of Luzhou-flavor liquor based on HS-SPME-GC-MS[J]. Journal of Light Industry, 2017, 32(6): 1-11. doi: 10.3969/j.issn.2096-1553.2017.6.001
Citation: HE Pei-xin, LI Cong-cong, HU Xiao-long, et al. Component analysis of volatile metabolites of culturable Clostridium spp. isolated from pit muds of Luzhou-flavor liquor based on HS-SPME-GC-MS[J]. Journal of Light Industry, 2017, 32(6): 1-11. doi: 10.3969/j.issn.2096-1553.2017.6.001 shu

Component analysis of volatile metabolites of culturable Clostridium spp. isolated from pit muds of Luzhou-flavor liquor based on HS-SPME-GC-MS

  • Corresponding author: HU Xiao-long, 
  • Received Date: 2017-08-30
  • A total of 88 pure cultures was obtained from the pit muds of one wine corporation following separation and purification culture. The 75 strains of bacteria were assigned into 8 phylogenetic clusters (cluster Ⅰ-Ⅷ) based on the analysis of 16S rRNA gene sequences and phylogenetic tree. In them, the most similar bacteria were C. tyrobutyricum, C. butyricum, C. indolis, C. acetobutylicum, C. bifermentans, C. sporogenes, C. cadaveris and C. kluyveri, respectively. The volatile metabolites of 8 representative Clostridium spp. were analyzed using headspace solid-phase microextraction (HS-SPME) method followed by gas chromatography-mass spectrometry (GC-MS). A total of 75 volatile metabolites was detected, including 23 alcohols,20 acids,14 esters,2 aldehydes,2 ketones and other 14 volatile substances. The principal component analysis revealed that the representative strain RL1 (C. tyrobutyricum) and EL7 (C. butyricum) were mainly related to the generation of acetic acid and butyric acid, and EL46 (C. kluyveri), RL20 (C. acetobutylicum) and RL10 (C. indolis) mainly contributed to the formation of caproic acid and ethyl hexanoate, and EL12 (C. bifermentans) and RL23 (C. sporogenes) were mainly associated with the S-methyl acetate, 1-Decyl alcohol and octanol, and EL43 (C. cadaveris) was mainly correlated to the biosynthesis of various esters.
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