大曲产酯化酶芽孢菌的分离鉴定及其发酵条件优化研究

侯小歌; 张杰; 孙忠科; 李童; 李学思; 闫培勋; 胡炳义

doi:10.3969/j.issn.2096-1553.2018.04.002

July 2018

Article Contents

HOU Xiaoge, ZHANG Jie, SUN Zhongke, et al. Isolation, identification and fermentation conditions optimization of an esterase-producing Bacillus strain from Daqu[J]. Journal of Light Industry, 2018, 33(4): 13-20. doi: 10.3969/j.issn.2096-1553.2018.04.002

Citation: HOU Xiaoge, ZHANG Jie, SUN Zhongke, et al. Isolation, identification and fermentation conditions optimization of an esterase-producing Bacillus strain from Daqu[J]. Journal of Light Industry, 2018, 33(4): 13-20. doi: 10.3969/j.issn.2096-1553.2018.04.002 shu

Isolation, identification and fermentation conditions optimization of an esterase-producing Bacillus strain from Daqu

HOU Xiaoge ¹ ,
ZHANG Jie ¹ ,
SUN Zhongke ¹ ,
LI Tong ¹ ,
LI Xuesi ² ,
YAN Peixun ² ,
HU Bingyi ¹

College of Life Science and Agriculture, Zhoukou Normal University, Zhoukou 466001, China;
He'nan Songhe Distillery Co., Ltd., Luyi 477265, China

Received Date: 2018-05-17
Available Online: 2018-07-15

Abstract

Using Songhe Daqu as the separation source, the spores were enriched and isolated by heating and dilution coating method. The esterase-producing strains were screened by transparent circle method and flask shaking fermentation method. Combined with morphology, physiological and biochemical characteristics and 16S rDNA sequence analysis, the high-yield esterase-producing Bacillus strain was initially identified and its fermentation conditions were optimized.A total of 81 strains of esterase-producing strains were screened and a high-yield esterase-producing strain SPEO-28 was screened out, which was initially identified as Bacillus licheniformis. The optimal esterification enzyme fermentation conditions of this strain were as follows:starch as carbon source and NH₄NO₃ as nitrogen source, initial pH value of 6.0, fermentation at 47℃ for 3 d. Under this condition, the esterase activity of the strain reached 22.83 U/mL, which was 82.6% higher than that before optimization.
- esterase-producing Bacillus strain,
- Daqu,
- esterifying enzyme,
- isolation and identification,
- fermentation condition optimization
References
1. [1]
  李大和,刘念,李国红.浓香型大曲酿造中酯化菌研究的现状与展望[J].酿酒科技,2008(2):92.
2. [2]
  张楷正,黄海.浓香型白酒酿造中的酯化酶研究及应用进展[J].酿酒科技,2016(2):93.
3. [3]
  滕巍,李国莹,刘小波,等.大曲中产酯化酶菌株的分离鉴定及固体发酵工艺优化[J].食品与生物技术学报,2016,35(9):971.
4. [4]
  XU Y,WANG D, FAN W,et al.Traditional Chinese biotechnology[J].Adv Biochem Eng Biot,2010,122:189.
5. [5]
  ZHENG X,TABRIZI M R,ROBERT NOUT M J,et al.Daqu:A traditional Chinese liquor fermentation starter[J].J Inst Brew,2011,117:82.
6. [6]
  范文来,徐岩.大曲酶系研究的回顾与展望[J].酿酒,2000(5):21.
7. [7]
  侯小歌,王俊英,李学思,等.浓香型白酒糟醅及窖泥产香功能菌的研究进展[J].微生物学通报,2013,40(7):1257.
8. [8]
  黄丹,方春玉,储玉龙,等.一株酯化酶细菌的分离、鉴定及代谢产物特征[J].四川理工学院学报(自然科学版),2010,23(3):321.
9. [9]
  YAN Z,ZHENG X,CHEN J,et al.Effect of different Bacillus strain on the profile of organic acids in a liquid culture Daqu[J].Institute of Brewing and Distilling,2013,119:78.
10. [10]
  WU Q,XU Y.Transcriptome profiling of heat-resistant strain Bacillus licheniformis CGMCC3962 producing Maotai flavor[J].J Agric Food Chem,2012,60(8):2033.
11. [11]
  WANG C L,SHI D J,GONG G L.Microorganisms in Daqu:A starter culture of Chinese Maotai-flavor liquor[J].World J Microbiol Biotechnol,2008,24:2183.
12. [12]
  LI X R,MA E B,YAN L Z,et al.Bacterial and fungal diversity in the starter production process of fen liquor, a traditional Chinese liquor[J].Journal of Microbiology,2013,51(4):431.
13. [13]
  蔡信之,黄君红.微生物学实验[M].北京:科学出版社,2010:203.
14. [14]
  张纪忠.微生物分类学[M].上海:复旦大学出版社,1990:67.
15. [15]
  鄢洪德,汪钊,李光伟.产酯酶微生物菌种的筛选研究[J].工业微生物,2007,37(6):44.
16. [16]
  刘阳.中高温大曲中产酯化酶细菌的选育[D].合肥:安徽工程大学,2013:27.
17. [17]
  ATESLIER Z B B,METIN K.Production and partial characterization of novel thermostable esterase from a thermophilic Bacillus sp.[J].Enzyme and Microbial Technology,2006,38(5):628.
18. [18]
  黄丹,张强,严芳,等.浓香型大曲中酯化酶细菌的分离鉴定及产酶条件研究[J].中国酿造,2009(8):57.
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沈阳化工大学材料科学与工程学院沈阳 110142

Isolation, identification and fermentation conditions optimization of an esterase-producing Bacillus strain from Daqu

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