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CN 41-1437/TS  ISSN 2096-1553

以碳量子点为荧光信号的生物传感器构建及其在金银花 Pb2+ 检测中的应用

李浩佳 ,  贺诗华 ,  曹艺泽 ,  郭西玉 ,  朱由余 ,  赵玮钦 ,  黄淳

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以碳量子点为荧光信号的生物传感器构建及其在金银花 Pb2+ 检测中的应用

  • 1. 西安科技大学 化学与化工学院, 陕西 西安 710054;

  • 2. 国土资源部煤炭资源勘查与综合利用重点实验室, 陕西 西安 710054

    • 作者简介: 李浩佳(1999—),女,河南省郑州市人,西安科技大学硕士研究生,主要研究方向为分析化学。E-mail:lhj16369909@163.com;
    通讯作者: 赵玮钦,wqzhao@xust.edu.cn

  • 基金项目: 国家自然科学基金青年基金资助项目(52102051)

  • 中图分类号: TS207.3

The construction of biosensor with carbon quan-tum dots as fluorescence signal and its application in detecting Pb2+ in honeysuckle

  • 1. School of Chemistry and Chemical Engineering, Xi'an University of Science and Technology, Xi'an 710054, China;

  • 2. Key Laboratory of Coal Resources Exploration and Comprehensive Utilization, Ministry of Land and Resources, Xi'an 710054, China

    • Corresponding author: ZHAO Weiqin, wqzhao@xust.edu.cn
  • Received Date: 2024-03-06
    Accepted Date: 2024-05-22
    Available Online: 2024-11-22

    CLC number: TS207.3

  • 摘要: 以葡萄柚皮为原料,采用水热法制备碳量子点(G-CQDs),并以此构建一种用于金银花中 Pb2+ 检测的荧光生物传感器。利用圆二色光谱、透射电子显微镜、紫外-可见吸收光谱、荧光光谱等对G-CODs的形貌和光学性能进行表征,优化该荧光生物传感器的实验条件,并对其检测性能进行分析。结果表明:G-CQDs呈球形且分布均匀,平均粒径为2.41 nm,最佳激发波长为350 nm;荧光生物传感器的适宜实验条件为Hemin浓度60 μmol/L、反应时间30 min、反应温度25℃、K+浓度10 mmol/L和pH值为6.5;与其他金属离子(Ag+、Zn2+、Cd2+、Cu2+和Hg2+)相比,荧光生物传感器对 Pb2+ 的选择性更高,且其Δ荧光强度随着 Pb2+ 质量浓度的增加而增强,在0.1~5.0 μg/mL线性范围内,二者线性关系良好,R2为0.998 0,检测限为0.063 9 μg/mL;与传统方法相比,该荧光生物传感器的准确性良好,且具有绿色环保、经济有效、操作简单等优势。
    Abstract: Grapefruit peel was used as raw material, and carbon quantum dots (G-CQDs) were prepared by hydrothermal method to construct a green and simple fluorescence biosensor for Pb2+ detection in honeysuckle. The morphology, structure and optical properties of G-CODs were characterized by circular dichroism spectroscopy, transmission electron microscopy,ultraviolet-visible absorption spectroscopy and fluorescence spectroscopy. The experimental conditions of the fluorescent biosensor were optimized and its detection performance was analyzed. The results showed that the G-CQDs were spherical and uniformly distributed. The average particle size was 2. 41 nm, and the optimal excitation wavelength was 350 nm. The suitable experimental conditions for the fluorescence biosensor were as follows: Hemin concentration of 60 μmol / L, reaction time of 30 min, reaction temperature of 25℃, K+ concentration of 10 mmol / L and pH value of 6. 5. Compared with other metal ions (Ag+, Zn2+, Cd2+, Cu2+ and Hg2+), the fluorescence biosensor had higher selectivity for Pb2+, and its fluorescence intensity increased with the increase of Pb2+ mass concentration. In the linear range of 0. 1 ~ 5. 0 μg / mL, the linear relationship between the two was good, R2 was 0. 998 0, and the detection limit was 0. 063 9 μg / mL. Compared with the conventional method (ICP-MS method), the fluorescence biosensor had good accuracy, and had the advantages of green environmental protection, economical and effective, simple operation and so on.
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  • 通讯作者:  赵玮钦, wqzhao@xust.edu.cn
  • 收稿日期:  2024-03-06
  • 修回日期:  2024-05-22
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以碳量子点为荧光信号的生物传感器构建及其在金银花 Pb2+ 检测中的应用

    作者简介:李浩佳(1999—),女,河南省郑州市人,西安科技大学硕士研究生,主要研究方向为分析化学。E-mail:lhj16369909@163.com
    通讯作者: 赵玮钦, wqzhao@xust.edu.cn
  • 1. 西安科技大学 化学与化工学院, 陕西 西安 710054;
  • 2. 国土资源部煤炭资源勘查与综合利用重点实验室, 陕西 西安 710054
  • 收稿日期: 2024-03-06
  • 录用日期: 2024-05-22
  • 网络出版日期: 2024-11-22
基金项目:  国家自然科学基金青年基金资助项目(52102051)

摘要: 以葡萄柚皮为原料,采用水热法制备碳量子点(G-CQDs),并以此构建一种用于金银花中 Pb2+ 检测的荧光生物传感器。利用圆二色光谱、透射电子显微镜、紫外-可见吸收光谱、荧光光谱等对G-CODs的形貌和光学性能进行表征,优化该荧光生物传感器的实验条件,并对其检测性能进行分析。结果表明:G-CQDs呈球形且分布均匀,平均粒径为2.41 nm,最佳激发波长为350 nm;荧光生物传感器的适宜实验条件为Hemin浓度60 μmol/L、反应时间30 min、反应温度25℃、K+浓度10 mmol/L和pH值为6.5;与其他金属离子(Ag+、Zn2+、Cd2+、Cu2+和Hg2+)相比,荧光生物传感器对 Pb2+ 的选择性更高,且其Δ荧光强度随着 Pb2+ 质量浓度的增加而增强,在0.1~5.0 μg/mL线性范围内,二者线性关系良好,R2为0.998 0,检测限为0.063 9 μg/mL;与传统方法相比,该荧光生物传感器的准确性良好,且具有绿色环保、经济有效、操作简单等优势。

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