以碳量子点为荧光信号的生物传感器构建及其在金银花 Pb<sup>2+</sup> 检测中的应用

李浩佳; 贺诗华; 曹艺泽; 郭西玉; 朱由余; 赵玮钦; 黄淳

Article Contents

Citation: . shu

The construction of biosensor with carbon quan-tum dots as fluorescence signal and its application in detecting Pb²⁺ in honeysuckle

LI Haojia ¹ ,
HE Shihua ¹ ,
CAO Yize ¹ ,
GUO Xiyu ¹ ,
ZHU Youyu ¹ ,
ZHAO Weiqin ^1,2,, ,
HUANG Chun ¹

1. School of Chemistry and Chemical Engineering, Xi'an University of Science and Technology, Xi'an 710054, China;
2. Key Laboratory of Coal Resources Exploration and Comprehensive Utilization, Ministry of Land and Resources, Xi'an 710054, China

Corresponding author: ZHAO Weiqin, wqzhao@xust.edu.cn
Received Date: 2024-03-06
Accepted Date: 2024-05-22
Available Online: 2024-11-22

Abstract

Grapefruit peel was used as raw material, and carbon quantum dots (G-CQDs) were prepared by hydrothermal method to construct a green and simple fluorescence biosensor for Pb²⁺ detection in honeysuckle. The morphology, structure and optical properties of G-CODs were characterized by circular dichroism spectroscopy, transmission electron microscopy,ultraviolet-visible absorption spectroscopy and fluorescence spectroscopy. The experimental conditions of the fluorescent biosensor were optimized and its detection performance was analyzed. The results showed that the G-CQDs were spherical and uniformly distributed. The average particle size was 2. 41 nm, and the optimal excitation wavelength was 350 nm. The suitable experimental conditions for the fluorescence biosensor were as follows: Hemin concentration of 60 μmol / L, reaction time of 30 min, reaction temperature of 25℃, K⁺ concentration of 10 mmol / L and pH value of 6. 5. Compared with other metal ions (Ag⁺, Zn²⁺, Cd²⁺, Cu²⁺ and Hg²⁺), the fluorescence biosensor had higher selectivity for Pb²⁺, and its fluorescence intensity increased with the increase of Pb²⁺ mass concentration. In the linear range of 0. 1 ~ 5. 0 μg / mL, the linear relationship between the two was good, R₂ was 0. 998 0, and the detection limit was 0. 063 9 μg / mL. Compared with the conventional method (ICP-MS method), the fluorescence biosensor had good accuracy, and had the advantages of green environmental protection, economical and effective, simple operation and so on.
- fluorescent biosensor,
- carbon quantum dot,
- DNAzyme,
- G-quadruplex,
- honeysuckle,
- lead ion
References
1. [1]
  王懿文,谢纯良,朱作华,等. 黑曲霉固态发酵对金银花多酚类物质释放及增效作用[J]. 食品研究与开发, 2023,44(22):23-29.
2. [2]
  王林,元丽娇,闵小莹,等. 金银花及其提取物在畜禽生产中的营养调节作用和潜在应用前景[ J]. 饲料研究,2023:1-8.
3. [3]
  张松,刘晓媛,吴迪. 6种药食同源中药材重金属及健康风险评价[ J]. 贵州师范大学学报(自然科学版), 2020,38(6). 33-38.
4. [4]
  MOHANRAJ P,BHUVANESHWARI S,SREELEKSHMIM S,et al. Bio-modified carbon paste electrode for the detection of Pb(Ⅱ) ions in wastewater[ J]. Water Scienge and Technology, 2019,80(11);2058-2066.
5. [5]
  ZORER ÇELEBI, EKIN Z,SELÇUK ZORER Ö. Accumulation and tolerance of Pb in Some Bioenergy crops[ J]. Polish Journal of Environmental Studies, 2018, 27(2): 591-596.
6. [6]
  LIU X Q,LI D D,LI J G,et al. A novel MnOx-impregnated on peanut shells derived biochar for high adsorption performance of Pb(Ⅱ) and Cd(Ⅱ):Behavior and mechanism[J]. Surfaces and Interfaces,2022,34:102323.
7. [7]
  FERNÁNDEZ Z H, VALCÁRCEL ROJAS L A, ÁLVAREZ A M, et al. Application of cold vapor-atomic absorption (CVAAS) spectrophotometry and Inductively coupled plasma-atomic emission spectrometry methods for cadmium,mercury and lead analyses of fish samples. Validation of the method of CVAAS[ J]. Food Control,2015, 48:37-42.
8. [8]
  DASILVA E,DAVID A M,PEJOVI-MILI A. The quantification of total lead in lipstick specimens by total reflection X-ray fluorescence spectrometry [ J]. X-Ray Spectrometry,2015,44(6):451-457.
9. [9]
  BORGGRÄFE J,VICTOR J,ROSENBACH H,et al. Timeresolved structural analysis of an RNA-cleaving DNA catalyst[J]. Nature,2022,601(7891):144-149.
10. [10]
  YUN W, CAI D Z, JIANG J L, et al. Enzyme-free and label-free ultra-sensitive colorimetric detection of Pb²⁺ using molecular beacon and DNAzyme based amplification strategy [ J ]. Biosensors and Bioelectronics, 2016, 80: 187-193.
11. [11]
  LI Y Y,LI H D,FANG W K,et al. Amplification of the fluorescence signal with clustered regularly interspaced short palindromic repeats-Cas12a based on Au nanoparticle-DNAzyme probe and on-site detection of Pb²⁺ via the photonic crystal chip [ J ]. ACS Sensors, 2022, 7(5): 1572-1580.
12. [12]
  RAVIKUMAR A,PANNEERSELVAM P,RADHAKRISHNAN K, et al. DNAzyme based amplified biosensor on ultrasensitive fluorescence detection of Pb (Ⅱ) ions from aqueous system[J]. Journal of Fluorescence,2017,27(6): 2101-2109.
13. [13]
  DOLATI S, RAMEZANI M, ABNOUS K, et al. Recent nucleic acid based biosensors for Pb²⁺ detection[ J]. Sensors and Actuators B: Chemical,2017,246:864-878.
14. [14]
  HUO X Z, HE Y X, MA S Q, et al. Green synthesis of carbon dots from grapefruit and its fluorescence enhancement [ J ]. Journal of Nanomaterials, 2020, 2020: 8601307.
15. [15]
  XIAO P, KE Y, LU J, et al. Photoluminescence immunoassay based on grapefruit peel-extracted carbon quantum dots encapsulated into silica nanospheres for p53 protein [ J ]. Biochemical Engineering Journal, 2018, 139: 109-116.
16. [16]
  伍永梅,朱肖倩,方娇,等. 基于改进G-四链体DNA酶的电化学适配体传感器构建及卡那霉素高灵敏检测[J]. 轻工学报,2023,38(6):62-69.
17. [17]
  DONG J T,O'HAGAN M P,WILLNER I. Switchable and dynamic G-quadruplexes and their applications [ J ]. Chemical Society Reviews,2022,51(17):7631-7661.
18. [18]
  ZHENG X T, ANANTHANARAYANAN A, LUO K Q, et al. Glowing graphene quantum dots and carbon dots: Properties, syntheses, and biological applications [ J ]. Small,2015,11(14):1620-1636.
19. [19]
  KUMARI B, KUMARI R, DAS P. Visual detection of G-quadruplex with mushroom derived highly fluorescent carbon quantum dots[ J]. Journal of Pharmaceutical and Biomedical Analysis,2018,157:137-144.
20. [20]
  ARHAM Z,KURNIAWAN K,ANHUSADAR L. High electrochemical response of TiO₂ @ C-dots nanocomposites as electrode modifiers for Pb (Ⅱ) detection[ J]. Materials Science in Semiconductor Processing,2023,160,107466.
21. [21]
  LI X H,ZHAO C X,LIN L L. Plasma-based instant synthesis of functionalized gold nanoparticles for colorimetric detection of lead ions[J]. Chemical Engineering Science, 2022,260,117849.
Proportional views