微生物发酵开发杏果渣香料的研究
Development of apricot pomace flavoring by microbial fermentation
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摘要: 为开发杏果渣香料,从杏果园土壤中分离筛选产香效果最佳的菌株,利用单因素试验和Box-Behnken响应面试验优化该菌株发酵杏果渣的工艺条件,并对其关键酶进行初步定位。结果表明:产香效果最佳的菌株LY13经鉴定为维克汉姆酵母菌(Wickerhamomyces),杏果渣经该菌株发酵后呈现丰富的甜香、果香和花香,且发酵液中醇类、酯类和酮类物质总量均较高;最佳发酵工艺条件为发酵转速145 r/min、发酵时间49 h、发酵温度31℃、初始pH值7.1,在此条件下,发酵产物中挥发性香气物质的总量为135.38 μg/g,且该菌株的产香关键酶主要为细胞膜酶。本研究可为产香微生物提供新型菌株资源,也为后续杏果渣发酵香料的工业化生产提供理论基础。Abstract: To develop apricot pomace flavoring, the most effective aroma-producing strain was isolated and screened from apricot orchard soil. The process conditions for microbial fermentation of apricot pomace by this strain were optimized using single-factor experiments and Box-Behnken response surface methodology, and the key enzyme was preliminarily identified. The results showed that the strain LY13, identified as Wickerhamomyces, had the best aroma-producing effect. After fermentation of apricot pomace by this strain, the resulting product exhibited rich sweet, fruity, and floral aromas, with a high total content of alcohols, esters, and ketones in the fermentation liquid. The optimal fermentation conditions were a stirring speed of 145 r / min, a fermentation time of 49 hours, a fermentation temperature of 31℃, and an initial pH of 7. 1. Under these conditions, the total content of volatile aromatic compounds in the fermented product reached 135. 38 μg / g, and the key enzyme responsible for aroma production was mainly located in the cell membrane. This study provides a novel strain resource for aroma-producing microorganisms and a theoretical basis for the industrial production of apricot pomace flavoring through microbial fermentation.
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Key words:
- apricot pomace /
- microbial fermentation /
- flavoring /
- key enzyme
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