豌豆-燕麦复合蛋白凝胶制备及配方优化研究
Investigation of the preparation and optimization of formulation for pea-oat complex protein gel
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摘要: 以豌豆-燕麦复合蛋白凝胶(POPG)为研究对象,以凝胶强度、持水性(WHC)、游离巯基含量、二硫键含量和微观结构为指标,考查不同酶(木瓜蛋白酶、胰蛋白酶、中性蛋白酶、转谷氨酰胺酶(TG酶)和漆酶)处理对POPG性质的影响,并以豌豆蛋白与燕麦蛋白复配比、复合蛋白溶液质量分数和TG酶添加量为因素,采用单因素和响应面试验优化凝胶配方。结果表明:TG酶添加量为40 U/g时,POPG表现出较好的凝胶强度和WHC,分别为83.00 g和88.79%,其游离巯基含量较低、二硫键含量较高、微观结构更为均匀;复合蛋白凝胶的最优配方为豌豆蛋白与燕麦蛋白复配比1 ∶ 0.4、复合蛋白溶液质量分数20%、TG酶添加量40 U/g,按此配方制备的复合蛋白凝胶表现出较好的凝胶强度(78.00 g)。本研究制备的POPG有望作为动物脂肪替代物,为后续绿色食品的加工提供参考。
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关键词:
- 豌豆-燕麦复合蛋白凝胶 /
- 酶处理 /
- 凝胶性质 /
- 配方优化
Abstract: The pea-oat complex protein gel (POPG) was taken as the research object, and the gel strength, water holding capacity (WHC), free sulfhydryl content, disulfide bond content and microstructure were cmeasured to investigate the effects of different enzymes (papain, trypsin, neutral protease, transglutaminase (TG enzyme) and laccase) treatment on the properties of POPG. The ratio of pea protein to oat protein, the mass fraction of complex protein solution and the amount of TG enzyme addition were usel as factors to optimize the formula of POPG by single factor and response surface test. When the addition of TG enzyme was 40 U / g, POPG showed good gel strength (83. 00 g) and WHC (88. 79%). Besides, the above POPG showed lower free sulfhydryl content, higher disulfide bond content, and uniform microstructure. The best formula of complex protein gel was that the ratio of pea protein to oat protein was 1 ∶ 0. 4, the mass fraction of the composite protein solution was 20%, and the TG enzyme addition was 40 U / g. The complex protein gel prepared according to this formula showed a good gel strength (78. 00 g). The POPG prepared in this study holds promise as an animal fat substitnte,offering insights for the processing of green food in the futnre.-
Key words:
- pea-oat complex protein gel /
- enzyme treatment /
- gel property /
- formula optimization
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