基于光胁迫粘红酵母合成高值化类胡萝卜素发酵条件优化
Optimization of fermentation conditions for high value carotenoid synthesis by Rhodotorula glutinis under light stress
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摘要: 以模拟日光照射为非生物环境胁迫条件,通过单因素试验和正交试验对粘红酵母(Rhodotorula glutinis)合成类胡萝卜素的发酵条件进行优化,研究光胁迫对粘红酵母生长和类胡萝卜素合成的影响。结果表明:光照强度为5000 Lux时,可显著提高粘红酵母生物量和类胡萝卜素产量;碳源对类胡萝卜素的合成影响最大,以甘油和葡萄糖最佳;最佳发酵条件为甘油质量浓度20 g/L、酵母粉质量浓度3.5 g/L、初始pH值5.0、培养温度25℃;5 L发酵罐培养144 h,生物量和类胡萝卜素产量分别达14.2 g/L和17.3 mg/L,比优化前分别提高了24.5%和21.0%;光胁迫在提高类胡萝卜素产量的同时,可有效提高高值化类胡萝卜素(红酵母红素和圆酵母素)在类胡萝卜素中的占比。Abstract: Taking simulated sunlight as abiotic environmental stress condition, the fermentation conditions of carotenoid synthesis by Rhodotorula glutinis were optimized by single factor test and orthogonal test. The effects of hight stress on growth of Rhodotorula glutinis and carotenoid synthesis were studied. The results showed that the light intensity of 5000 Lux could significantly increase the biomass and carotenoid yield. The type of carbon sources had the greatest effect on carotenoid biosynthesis. Glycerol and glucose could be better used to synthesize carotenoids. The optimal fermentation conditions were glycerol concentration of 20 g/L, yeast powder addition of 3.5 g/L, the initial pH value 5.0 and temperature of 25 ℃. After cultured in 5 L fermentor for 144 h, the biomass and carotenoid yield reached 14.2 g/L and 17.3 mg/L, respectively, which were 24.5% and 21.0% higher than those before optimization; Light stress could not only increase the yield of carotenoids, but also effectively increase the proportion of high value-added carotenoids (torularhodin and torulene) in the product.
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Key words:
- light stress /
- Rhodotorula glutinis /
- carotenoid /
- fermentation conditions /
- high value
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