基于高通量测序和纯培养联用技术的药曲微生物多样性解析
Analysis of microbial diversity in meidicinal koji based on high-throughput sequencing and pure culture techniques
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摘要: 采用MiSeq高通量测序和纯培养联用技术对采集自江西省南昌市的药曲样品进行微生物多样性解析,以揭示药曲中的微生物群落结构。结果表明:药曲中细菌类群的丰富度和多样性均明显高于真菌类群,细菌主要优势属为片球菌属(Pediococcus)和促生乳杆菌属(Levilactobacillus),平均相对含量分别为38.04%和13.00%,真菌优势属为假丝酵母属(Candida)和复膜孢酵母属(Saccharomycopsis),平均相对含量分别为78.30%和17.92%。从药曲中分离出的9株细菌被鉴定为戊糖片球菌(Pediococcus pentosaceus)、乳酸片球菌(Pediococcus acidilactici)和地衣芽孢杆菌(Bacillus licheniformis)3个种,7株真菌被鉴定为扣囊复膜酵母(Saccharomycopsis fibuligera)、马兰加酵母(Saccharomycopsis malanga)、库德里阿兹威毕赤酵母(Pichia kudriavzevii)和酿酒酵母(Saccharomyces cerevisiae)4个种。
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关键词:
- 药曲 /
- MiSeq高通量测序 /
- 纯培养 /
- 微生物多样性
Abstract: MiSeq high-throughput sequencing and pure culture techniques were combined to analyze the microbial diversity of medicinal koji samples collected from Nanchang city, Jiangxi province, aiming to reveal the microbial composition within the koji. The results showed that the richness and diversity of bacterial taxa in the medicinal koji included in this study were significantly higher than those of the fungus. The dominant bacterial genera were Pediococcus and Levilactobacillus, with an average relative content of 38. 04% and 13. 00%, respectively. The dominant fungus genera were Candida and Saccharomycopsis, with an average relative content of 78. 30% and 17. 92%, respectively. Nine strains of bacteria isolated from medicinal koji were identified as three species: Pediococcus pentosaceus, Pediococcus acidilactici, and Bacillus licheniformis. Seven strains of fungus were identified as four species: Saccharomycopsis fibuligera, Saccharomycopsis malanga, Pichia kudriavzevii, and Saccharomyces cerevisiae.-
Key words:
- medicinal koji /
- MiSeq high-throughput sequencing /
- pure culture /
- microbial diversity
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