JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

2012 Vol. 27, No. 5

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Recent advances of technique and method on the protein separation and identification in the proteomic studies
YANG Kai-guang, ZHANG Li-hua, ZHANG Yu-kui
2012, 27(5) :1-7,12. doi: 10.3969/j.issn.2095-476X.2012.05.001
Abstract:
The new techniques and methods of protein separation and identification in the program "Novel Techniques and Methods for Protein Separation and Identification",supported by "National Basic Research Program of China",were reviewed.For high-abundance proteins depletion,strong cation exchange (SCX) chromatography was coupled with reversed phase liquid chromatography (RPLC) to develop two dimensional liquid chromatography,and high abundance proteins were applied as the templates to developed molecularly imprinted materials.For low abundance protein enrichment selectively,novel functional materials were developed for selectively capturing significant post-translated proteins,such as phosphopeptides/proteins and glycopeptides/proteins.For multidimensional,multi-mode and array protein separation,column switch recycling size exclusion chromatography (csrSEC) was coupled with RPLC to develop multidimensional liquid chromatography,and weak anion and cation exchange chromatography mixed-bed microcolumn were integrated with the immobilized trypsin reactor and RPLC-ESI/MS/MS to develop at high-throughput proteome platform.During the study of high-sensitive identification techniques,an online integrated platform for sample pretreatment,which was based on the hollow fiber membrane interface for solvent exchange,was established.New reagents were developed for the peptide derivatization,which decreased the limit of detection of peptide on the mass spectrum by 10-100 times.Core-shell nanoparticles were employed on the plate materials directly for the matrix-assisted laser desorption/ionization mass (MALDI).A predictive genetic algorithm was implemented for the optimization of filtering criteria to maximize the number of identified peptides for mass spectrum database searching.The new technology and new method for proteomic study provides an effective method.The development of selective adsorption materials provides a new way for high abundance protein depletion and low abundance proteins enrichment of proteomics research,and avoids the interference of the target protein identification in complex system.The platform of multidimensional and mode,array type of protein efficient separation technology as the core is constructed and realized the protein group of high efficiency,high flux,high reliability separation.With the new technology and new method development of high performance liquid chromatography/mass spectrometry as the core of high sensitive identification,the sensitivity and accuracy of low abundance protein mass spectrum identification are improved.
Review of research advances on preparation and applications of amylomaltases from thermus scotoductu
LU Yong, WU Zong-shuai, HU Zhong-yu, LI Xue-hong
2012, 27(5) :8-12. doi: 10.3969/j.issn.2095-476X.2012.05.002
Abstract:
The production of amylomaltases and its promising applications were reviewed.The preparation was carried out through gene cloning to normal temperature host cell and the host cell excessive expression and the application reflected in modification of starches,production of large-ring cyclodextrins and transglycosylation of some functional ingredients,etc.It was further pointed out that the future research will focuse on multifunctional catalytic mechanism of the enzyme and increasing the varieties of glycosylation reactions as well as their functonal products.
Review of diagnosis methods and vaccine research of enterovirus 71
DONG Cai-wen, ZHANG Sheng-nan, WANG Yun-long, LI Yu-lin, SUN Xin-cheng, WANG Guo-qiang, LI Heng-si
2012, 27(5) :13-17. doi: 10.3969/j.issn.2095-476X.2012.05.003
Abstract:
The diagnosis method and vaccine research were summarized.There are several diagnosis methods for enterovirus 71 (EV71),such as separation and culture of virus,titer test of neutralizing antibody, nucleic acid detection and immunological assay.The main research contents of EV71 vaccine are attenuated live vaccine based on the C4 strain and evaluated through animal model, virus like particle vaccine and recombinant vaccine aimed at VP1 site.It is provided one effective method for rapid diagnosis of EV71 combined use of two or more diagnosis assay.The foundation of suitable animal model, the screening of virus strain with high immunogenicity and cross protection, and the comparison of immunogenicity for different vaccine are the direction of virus research.
Preparation of flavor vinegar jelly with kiwi and apple
XU Qing-ping, HU Feng-cheng
2012, 27(5) :18-20. doi: 10.3969/j.issn.2095-476X.2012.05.004
Abstract:
Vinegar,kiwi and apple were used for making flavor vinegar jelly.The influences of the dosage of jelly powder,complex juice,sweetener,and vinegar on flavor vinegar jelly were studied.The best ratio for fish powder,xanthan gum and agar was 1:1:2 and the best ratio for kiwifruit juice and apple juice was 3:1 according to experiment.The optimal ratio for flavor vinegar jelly,which was determined by orthogonal experiments,was as follows:jelly powder 1.0%,fruit juice 20%,sweetener 4.0% (the ratio for aspartame and granulated sugar was 1:50),white vinegar 6.0%.Vinegar jelly obtained was healthy with even color,good state,sour and sweet taste,and balanced smell.
The effect of chitosan on the preservation of fresh cut apple
ZHANG Wei-na, DAI Zeng-ying, FENG Jian-ling, LI Ying-qiu, YU Hui-hui
2012, 27(5) :21-24. doi: 10.3969/j.issn.2095-476X.2012.05.005
Abstract:
With fresh cut apple as the test raw material,different concentration chitosan solution was prepared with acetic acid of 1%.The fresh cut apple was preserved in 0℃ and 25℃ after daubing the fresh cut apple chitosan solution.Titratable acid,browning degrees,and loss ratio were measured in a certain interval of time.The results showed that the preservation effect was the best when chitosan concentration was 1.63% at 0℃ and 90 s.
Simultaneous determination by gas chromatographic-mass spectrometric analysis of plasticizers in plastic film assisted by continuous wavelet transform
WANG Guo-qing, YANG Wei-fang, WANG Su-fang, SHI Ming-xia, PANG Wen-yue, SUN Xiao-li, TAO Hai
2012, 27(5) :25-29. doi: 10.3969/j.issn.2095-476X.2012.05.006
Abstract:
Plasticizers in PVC plastic films were extracted by homogeneous-ultrasonic using hexane as the solvent.Using gas chromatography-mass spectrometry (GC-MS) determination of nine plasticizers in PVC films,the total ion current (TIC) chromatograms of diisononyl phthalate (DINP) and diisononyl phthalate (DINP) were seriously overlapped.For resolution of the overlapped TIC chromatograms of DINP and DIDP,continuous wavelet transform (CWT) was introduced.The results showed that they overlapped peaks were well separated.The proposed CWT assisted homogeneous-ultrasonic extraction and GC-MS analysis provides an alternative novel way for simultaneous determination of multiple plasticizers in PVC films.
Study on film formation feature of PP-g-MAH emulsion
TAO Ying-ying, DANG Jing-chuan, ZHAO Ke
2012, 27(5) :30-33. doi: 10.3969/j.issn.2095-476X.2012.05.007
Abstract:
DTA-TG analysis of PP-g-MAH emulsion in air and N2 was conducted in order to investigate its heat stability.The film formation time of different solid content PP-g-MAH emulsion film at different temperature and the contact angle to water,the hardness and coating adhesion of they were tested.The results showed that PP-g-MAH emulsion presented very good heat stability within 200℃.The contact angle and hardness of the film presented the trend of increasing firstly and then decreasing with the rise of temperature.And the latex film achieved the best comprehensive performance at the temperature of 165℃.
Review of extraction of natural polysaccharides and its application in the cigarette
XU Chun-ping, YANG Chen-chen, GAO Jian-qi, MAO Duo-bin
2012, 27(5) :34-37. doi: 10.3969/j.issn.2095-476X.2012.05.008
Abstract:
The research progress of natural polysaccharides extraction and its application in the cigarette were reviewed.The sources were mainly plant polysaccharides,fungal polysaccharides and seaweed polysaccharides.The extraction methods included solvent extraction,acid-base extraction and enzyme extraction.The natural polysaccharides could be added to the cigarette tobacco as a feed solution,or filter tip additive,or used in cigarette paper,or humectant to improve the cigarette smoke flavor,reduce irritation,and increase moisture retention.Extraction methods,assay of bioactive components and structural analysis,pyrolysis characteristics,and structure-function relationship would be the direction of development.
Effect of humectant on moisture retention of cigarette
ZHANG Li, ZHANG Xiang-hui, XU Li-xia, GUAN Ti-qing, TAO Ye, ZHANG Shuai, AN Yi
2012, 27(5) :38-40,58. doi: 10.3969/j.issn.2095-476X.2012.05.009
Abstract:
The monomer humectant and the additive monomer humectant were compared by moisture retention experiment,sensory comfort was evaluated by addition of humectant tobacco and proved in cigarette.The results showed that the monomer humectant honey was better than others,the additive monomer humectants honey-propylene glycol was better than others,the added monomer humectant and additive monomer humectant cigarette had the better sensory comfort,mainly for thrill and dry ensation were decreased,the taste was improved evidently.
Pyrolysis analysis of maple concrete and its application in cigarette
SU Dong-ying
2012, 27(5) :41-45. doi: 10.3969/j.issn.2095-476X.2012.05.010
Abstract:
The pyrolysis of maple concrete was performed by an on-line pyrolysis gas chromatography/mass spectrometry (Py-GC-MS) for simulation cigarette combustion process.The maple concrete was pyrolyzed at different temperatures (300℃,600℃,900℃); the pyrolysis products were directly introduced into GC-MS to make,qualitative and quantative analysis and cigarette flavoring experiments were carried out.The analysis results showed that the main pyrolysis products are aldehydes,ketones,esters and heterocyclic substances at different temperature,most of which have elegance flavor.The pyrolytic compounds were more and more complex with the increase of temperature.The tobacco flavoring experiment showed that the maple concrete added in the tobacco at 0.02% was appropriate,imparting a sweet,caramelic flavor to the cigarette and improving cigarette sensory quality.
The correlation analysis of sensory quality of burley tobacco and its chemical constituents under different roasting conditions
XU Li-xia, AN Yi, GUAN Ti-qing, YANG Jing, MAO Duo-bin
2012, 27(5) :46-50,54. doi: 10.3969/j.issn.2095-476X.2012.05.011
Abstract:
The influence of different roasting conditions on burley tobacco aroma compounds,chemical compounds and sensory quality was studied.The results showed that pyrazine compounds,pyridine compounds,the total content of basic aroma compounds and chemical compounds had significant correlation with sensory quality.By comparing the contents of chemical compounds with sensory quality of the burley tobacco,the optimum condition for burley tobacco roasting was established.Roasting temperature 120℃,air moisture 400 g/kg and final tobacco moisture content 8% were the most suitable conditions.
Extraction of lignin from tobacco stem with high boiling solvent
ZHENG Yong-jie, MA Lin, KOU Xiao-teng, ZHANG Rui
2012, 27(5) :51-54. doi: 10.3969/j.issn.2095-476X.2012.05.012
Abstract:
Lignin was extracted from the tobacco stem with aqueous solution of 1,4-butanediol as extracting reagent by high boiling extraction.The best extracting conditions were as follows:50 g desiccated tobacco stem,the volume ratio of 1,4-butanediol:water 9:1,the solid-liquid ratio(g:mL)1:10,a small amout of concentrated H2SO4 as catalyst,the extraction temperature 220℃,reaction time 2 h,and the yield was the highest.The chemical structure of the tobacco stem lignin was characterized by UV and FTIR.The results showed that lignin from tobacco stem using high boiling solvent had the typical characteristics of lignin compounds.
Effects of the vacuum conditioning process on qualities of the paper-making process reconstituted tobacco
GAO Jin-ling, LI Shu-fang, ZHU Guo-cheng, WEI Jia-xin
2012, 27(5) :55-58. doi: 10.3969/j.issn.2095-476X.2012.05.013
Abstract:
A contrastive analysis was chosen to analyse the sensory quality and processing quality of paper-making process reconstituted tobacco before and after vacuum.The cut tobacco structure,physical and chemical characteristics and the sensory quality of the finished tobacco product were also analysed after blending.The results showed that the smoking quality of the paper-making process reconstituted tobacco was improved after vacuum conditioning,but the aroma components lose;There was no significant effect on the tobacco structure,physical and chemical character istics and sensory quality of the finished cigarettes.
Temperature prediction and heat transfer model study of food product in vertical open refrigerated display cabinet
LV Yan-li, MENG Zhi-qiang, CAO Xiu-qin, MA Qiu-yang, WU Xue-hong, ZHANG Wen-hui
2012, 27(5) :59-62. doi: 10.3969/j.issn.2095-476X.2012.05.014
Abstract:
Heat transfer by conduction,convection and radiation was taken into account in an open vertical display cabinet.The load positions were divided into four regions for a heat transfer model.The heat transfer model simplified as a two-dimensional problem was analyzed and modified.Then it was used to predict the load temperature in an open vertical display cabinet.The result showed that predicted values basically fitted with the experiment results.The deviation of bottom/front and top/rear load temperature between model and experiment was larger.The front load temperature changed more than the rear with the ambient temperature increase.And the result was coincided with the fact.
The change law of remote sensing signal of rocket exhaust plume radiation with the variation of aviatic parameters
YIN Xue-mei, YAN Li-qiang, WU Xue-hong, GONG Yi, LV Yan-li
2012, 27(5) :63-67,77. doi: 10.3969/j.issn.2095-476X.2012.05.015
Abstract:
A wide band k-distribution model was established to calculate the remote sensing signal of rocket exhaust plume,the change law of remote sensing signal with variation of aviatic parameters was investigated,and the ratios of rocket exhaust plume's radiation signal were analyzed and compared.The results showed that between the 8.0~11.5 μm and 10.0~13.4 μm,the sensing integral radiation intensities increased with the increase of the nozzle exit temperature,Mach number and ratio of pressure and decreased with the altitude's increase.The own and sensing integral radiation intensities increased with the nozzle exit temperature,Mach number and altitude and decreased with the ratio's increase.
Study on shear behavior on honeycombed steel beam and welded compound-ring-hoop concrete column composite joint connected by extended end-plate
MA Yan-ru, LI Sheng-cai
2012, 27(5) :68-72. doi: 10.3969/j.issn.2095-476X.2012.05.016
Abstract:
The shear behaviour of honeycombed steel beam and welded compound-ring-hoop concrete column composite joint connected by extended end-plate through a low-frequency quasi-static cyclic loading experiment were investigated.The experimental results showed that because of the restriction effect of compound-ring-hoop,the shear capability of concrete in the joint region under three-directional pressure was significantly improved in contrast with the shear capability of concrete in the reinforced concrete joint region.Because of the pre-pressure of high-strength bolts,concrete cracks were fewer in the joint region and the shear behaviour of joint region was improved.
Study on composite exponential-hyperbolic normal deformation model of rock fractures
CAI Yan-yan, ZHENG Chun-ting, QI Zhi-bo, CHEN Xu, MU Kang
2012, 27(5) :73-77. doi: 10.3969/j.issn.2095-476X.2012.05.017
Abstract:
A parameter α was introduced into the new 3-parameter composite exponential-hyperbolic model on account of the defects of the conventional 2-parameter exponential model and BB model of the normal deformation of rock fractures.The new model got over the defects of the conventional models,i.e.exaggeration of dn under medium stress level by exponential model and contraction of dn with the low rate of approaching to dmax under medium stress level by BB model.The new model was validated by the testing data of grano diorite.And the validation showed that the simulation result from new model was superior to those from the BB model and exponential model,which provided a proof of feasibility and scientificity of the new model.
Experimental study on mechanical property of composite laminate cementing under different temperature
WANG Yi, JIANG Zhi-qiang, WANG Pei-yan
2012, 27(5) :78-80,106. doi: 10.3969/j.issn.2095-476X.2012.05.018
Abstract:
The tensile crippling mechanical property of composite laminate cementing(T100/T500) under three different temperatures were studied by experiment.The results of the experiment proved that the first crippling load of the high temperatures was only half of the room temperature specimens,the ultimate failure load reached 1.79 fold of the room temperature specimens,and the average maximum load of the low temperatures specimens was 72.64% of the room temperature specimens.The mechanical property of the cementing (T100/T500) under the high temperature was superior to the others.
Weak signal detection based on Duffing oscillator
SUN Yu-sheng, ZHAN Xiao-xia, SHI Jun, HU Zhi-hong
2012, 27(5) :81-84. doi: 10.3969/j.issn.2095-476X.2012.05.019
Abstract:
Aiming at the problem that it was difficult to visual test phase trajectories form as a law intermittency phenomenon,and which could not be used in engineering practice directly.The phase trajectories was identified through Hu type invariant moment as quantitative indicator in the detection of weak signal characteristics based on the Duffing oscillator.A rapid and accurate quantitative measure was obtained to facilitate the automatical recognization of computer which could be applied to engineering practice.
Modeling and simulating analysis for software radio system
TIAN Yong-yi
2012, 27(5) :85-88. doi: 10.3969/j.issn.2095-476X.2012.05.020
Abstract:
Aiming at the problem between system performance and design complexity in the traditional software radio system,a new model was made by cascade filtering method.Based on this model,the validity of transmission and system reliability were analysed by computer simulation on Matlab.The simulation results showed that the model could reduce design complexity on the premise of specified performance.
Design on a monitoring system of rural power grids based on GPRS
ZHANG Jin-song
2012, 27(5) :89-91. doi: 10.3969/j.issn.2095-476X.2012.05.021
Abstract:
A monitoring system of rural power grids based on GPRS was designed.In this system,the difference of frequence and phase was measured via the internal timer of MCU STC12C5410AD.The normal running information and fault information through GPRS wireless communication module would be sent to data center by instantaneous sample of effective value voltage and current to cooperate with sampling,magnifying,precision rectifier and filter circuit.The system had features such as small volume,low price,low power consumption,convenience,stability and reliability.It would be an effective solution to remote monitoring of parameters of power system.
Knowledge representation of emergency knowledge base based on Ontology
JIN Bao-hua, LIN Qing, FU Zhong-ju
2012, 27(5) :92-94,98. doi: 10.3969/j.issn.2095-476X.2012.05.022
Abstract:
To solve the problem of establishment of emergency knowledge base and stuctural knowledge representation,the emergency knowledge base model based on Ontology was designed.RDF/OWL technology and Protégé ontology editor tool were used to establish emergency knowledge base,the semantic model of emergency knowledge base was designed based on Ontology.The result showed that the research provided great technical support to knowledge representation in emergency knowledge base.Moreover,it also layed the foundation to the following reasoning work.
Simulation modeling and implementation of two-dimensional enclosed space sound propagation system
FENG Yuan, CAI Zeng-yu, GAN Yong, LI Na-na
2012, 27(5) :95-98. doi: 10.3969/j.issn.2095-476X.2012.05.023
Abstract:
Aiming at the difficulty in actual measurement of sound propagation,the two-dimensional enclosed space model of sound propagation system based on Maxwell's wave equation was established,the wave equation was discretized using Nyquist theory.The simulation arithmetic of two-dimensional enclosed space sound propagation system was built and realized using Matlab.The results showed that it can intuitively show of sound in a enclosed space condition, record enclosed space acoustic field each point changing with time, and it is easy to realize for two-dimensional enclosed space sound propagation process observation and measurement.
Carrier frequency offset estimation for OFDM in highly mobile channels
CHEN Dong-hua
2012, 27(5) :99-102. doi: 10.3969/j.issn.2095-476X.2012.05.024
Abstract:
In order to improve the estimation performance of carrier frequency offset (CFO) for OFDM systems in time-varying channel,the short repetitive training sequences were inserted between any two OFDM symbols and then were used to estimate the CFO.The impact of time-varying channel on the estimation of CFO was thus reduced by decreasing the correlation interval.Simulation results showed that when the normalized Doppler frequency of time-varying channel was above 0.1,the short training sequence based method was superior in performance to the conventional cyclic prefix based one,especially so when the channel varies faster,and thus was more applicable in high mobility environments.
The H control for discrete-time fuzzy bilinear switched system
MAO Bei-xing, ZHOU Chang-qin, BU Chun-xia
2012, 27(5) :103-106. doi: 10.3969/j.issn.2095-476X.2012.05.025
Abstract:
The H control problem of a kind of discrete-time fuzzy bilinear switched systems based on T-S model was studied.The stable condition and switching laws design method were concluded based on Lyapunov function.The conclusion was given in linear matrix inequality.The method validity was testified through numerical example.
A property of quaternion self-conjugate matrix
XU Ji-jun, REN Xi-feng
2012, 27(5) :107-108. doi: 10.3969/j.issn.2095-476X.2012.05.026
Abstract:
A property of quaternion self-conjugate matrix was discussed.The definition of n order quaternion positive definite (positive semi-definite,negative definite)self-conjugate matrix was simplified using this property and the same conclusions with quaternion self-conjugate matrix were drawn.
Journal Information

Founded in 1986, bimonthly

Administered by:The Education Department Henan Province

Sponsored by:Zhengzhou University of Light Industry

Editor-in-chief:Wei Shizhong

Executive Editor-in-Chief:Zou Lin

Subeditor:Qu Shuanghong

Edited & published by:Editorial Department of Journal of Light Industry

CN 41-1437/TS

ISSN 2096-1553

Address:136 Science Avenue, Zhengzhou City, Henan Province, China

Postal Code:450001

Tel:(086)0371-86608635
(086)0371-86608633

Email:qgxb@zzuli.edu.cn
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