基于HPLC指纹图谱与多元统计分析的太平猴魁产地判别方法
Origin discrimination of Taiping Houkui based on HPLC fingerprints and multivariate statistical analysis
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摘要: 以核心产地和非核心产地的太平猴魁为研究对象，通过优化高效液相色谱（HPLC）检测方法建立太平猴魁HPLC指纹图谱，并结合多元统计分析方法对不同产地太平猴魁样品进行鉴别分析。结果表明：按优化后的HPLC检测方法对核心产地10种太平猴魁样品进行定量分析，共鉴定出11个共有峰（GA、5-GC、GC、TB、EGC、Cat、EC、Caf、EGCG、GCG、ECG），且10种太平猴魁样品的相似度均在0.982以上；来自核心产地与非核心产地的太平猴魁样品在PCA得分图中按照产地聚集，实现了较好的分离；筛选出的6种生物标志物（EC、GC、Cat、TB、EGC、Caf）可作为区分太平猴魁是否来自核心产地的特征性成分。Abstract: Taiping Houkui from core and non-core producing areas was taken as the research object. The HPLC fingerprints of Taiping Houkui was established by optimizing high performance liquid chromatography (HPLC) detection methods, and the multivariate statistical methods were used to analyze Taiping Houkui from different producing areas. The results showed that according to the optimized HPLC detection method, the 10 kinds of Taiping Houkui samples from the core producing area were quantitatively analyzed, and 11 common peaks(GA、5-GC、GC、TB、EGC、Cat、EC、Caf、EGCG、GCG、ECG) were identified and well separated, and the similarity of 10 Taiping Houkui samples was above 0.982. Taiping Houkui samples from core and non-core producing areas were clustered together according to the origin in the PCA score chart. The 6 biomarkers (EC, GC, Cat, TB, EGC, Caf) screened out were characteristic components that could distinguish whether Taiping Houkui came from the core producing area.
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Key words:
- Taiping Houkui /
- HPLC /
- fringerprints /
- multivariate statistical analysis
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