JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

代谢工程改造枯草芽孢杆菌促进L-赖氨酸高效合成研究

张晓霞 ,  陈胜玲 ,  朱枝群 ,  康春涛 ,  徐建中

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张晓霞, 陈胜玲, 朱枝群, 等. 代谢工程改造枯草芽孢杆菌促进L-赖氨酸高效合成研究[J]. 轻工学报, 2022, 37(5): 1-11. doi: 10.12187/2022.05.001
引用本文: 张晓霞, 陈胜玲, 朱枝群, 等. 代谢工程改造枯草芽孢杆菌促进L-赖氨酸高效合成研究[J]. 轻工学报, 2022, 37(5): 1-11. doi: 10.12187/2022.05.001
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ZHANG Xiaoxia, CHEN Shengling, ZHU Zhiqun, et al. Metabolic engineering for improving the L-lysine production by Bacillus subtilis[J]. Journal of Light Industry, 2022, 37(5): 1-11. doi: 10.12187/2022.05.001
Citation: ZHANG Xiaoxia, CHEN Shengling, ZHU Zhiqun, et al. Metabolic engineering for improving the L-lysine production by Bacillus subtilis[J]. Journal of Light Industry, 2022, 37(5): 1-11. doi: 10.12187/2022.05.001
shu

代谢工程改造枯草芽孢杆菌促进L-赖氨酸高效合成研究

  • 1. 江苏星海生物科技有限公司, 江苏 盐城 224233;

  • 2. 江南大学 生物工程学院/工业生物技术教育部重点实验室, 江苏 无锡 214122

    • 作者简介: 张晓霞(1980-),女,江苏省东台市人,江苏星海生物科技有限公司工程师,主要研究方向为发酵工程。E-mail:aixuexi_2016@163.com;

  • 基金项目: 苏北科技专项-先导性项目(SZ-YC202105)

  • 中图分类号: TS264

Metabolic engineering for improving the L-lysine production by Bacillus subtilis

  • 1. Jiangsu Xinghai Biotechnology Co., Ltd., YanCheng 224233, China;

  • 2. School of Biotechnology/The Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University, WuXi 214122, China

  • Received Date: 2022-02-13

    CLC number: TS264

  • 摘要: 为了构建具有益生功能和L-赖氨酸合成功能的“双功能”枯草芽孢杆菌(Bacillus subtilis)重组菌株,对饲料工业常用的益生菌B.subtilis ACCC11025进行系统的代谢工程改造。结果表明:以来源于谷氨酸棒杆菌(Corynebacterium glutamicum)的lysC311、zwf234和gnd361替换B.subtilis中的thrD、zwf和gnd,即构建重组菌B.subtilis XH4,有利于L-赖氨酸的合成,其产量达到(20.3±1.9) g/L;将B.subtilis中hom替换成来源于C.glutamicum的hom59,即构建重组菌B.subtilis XH5,可显著降低副产物积累量,提高L-赖氨酸产量至(23.2±1.7) g/L,且不影响菌体生长;在重组菌B.subtilis XH5中引入C.glutamicum中的DapDH会改变二氨基庚二酸途径(DAP)碳分布进而促进L-赖氨酸的合成,目标重组菌B.subtilis XH6的L-赖氨酸产量达到(25.6±2.3) g/L。
    Abstract: In order to construct a dual-functional B.subtilis with probiotic function and L-lysine production, B.subtilis ACCC11025 was systematically modified. These results indicated that the strain with replacement of thrD,zwf and gnd from B.subtilis by lysC311,zwf234 and gnd361 from C.glutamicum (i.e.,B.subtilis XH4) was beneficial to L-lysine production, and the yield of L-lysine was (20.3±1.9) g/L. In addition, the strain with replacement of hom from B.subtilis by hom59 from C.glutamicum(i.e.,B.subtilis XH5)produced (23.2±1.7) g/L of L-lysine without the decrease of cell growth. In addition, the yield of by-products in B.subtilis XH5 was significantly decreased. Moreover, the DapDH from C.glutamicum was introduced into the B.subtilis XH5 (i.e, B.subtilis XH6), resulting in the increase of L-lysine production because of the redirection of the carbon flux in DAP pathway. The resulted recombinant strain B.subtilis XH6 produced (25.6±2.3) g/L of L-lysine.
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张晓霞, 陈胜玲, 朱枝群, 等. 代谢工程改造枯草芽孢杆菌促进L-赖氨酸高效合成研究[J]. 轻工学报, 2022, 37(5): 1-11. doi: 10.12187/2022.05.001
引用本文: 张晓霞, 陈胜玲, 朱枝群, 等. 代谢工程改造枯草芽孢杆菌促进L-赖氨酸高效合成研究[J]. 轻工学报, 2022, 37(5): 1-11. doi: 10.12187/2022.05.001
shu
ZHANG Xiaoxia, CHEN Shengling, ZHU Zhiqun, et al. Metabolic engineering for improving the L-lysine production by Bacillus subtilis[J]. Journal of Light Industry, 2022, 37(5): 1-11. doi: 10.12187/2022.05.001
Citation: ZHANG Xiaoxia, CHEN Shengling, ZHU Zhiqun, et al. Metabolic engineering for improving the L-lysine production by Bacillus subtilis[J]. Journal of Light Industry, 2022, 37(5): 1-11. doi: 10.12187/2022.05.001
shu

代谢工程改造枯草芽孢杆菌促进L-赖氨酸高效合成研究

    作者简介:张晓霞(1980-),女,江苏省东台市人,江苏星海生物科技有限公司工程师,主要研究方向为发酵工程。E-mail:aixuexi_2016@163.com
  • 1. 江苏星海生物科技有限公司, 江苏 盐城 224233;
  • 2. 江南大学 生物工程学院/工业生物技术教育部重点实验室, 江苏 无锡 214122
  • 收稿日期: 2022-02-13
基金项目:  苏北科技专项-先导性项目(SZ-YC202105)

摘要: 为了构建具有益生功能和L-赖氨酸合成功能的“双功能”枯草芽孢杆菌(Bacillus subtilis)重组菌株,对饲料工业常用的益生菌B.subtilis ACCC11025进行系统的代谢工程改造。结果表明:以来源于谷氨酸棒杆菌(Corynebacterium glutamicum)的lysC311、zwf234和gnd361替换B.subtilis中的thrD、zwf和gnd,即构建重组菌B.subtilis XH4,有利于L-赖氨酸的合成,其产量达到(20.3±1.9) g/L;将B.subtilis中hom替换成来源于C.glutamicum的hom59,即构建重组菌B.subtilis XH5,可显著降低副产物积累量,提高L-赖氨酸产量至(23.2±1.7) g/L,且不影响菌体生长;在重组菌B.subtilis XH5中引入C.glutamicum中的DapDH会改变二氨基庚二酸途径(DAP)碳分布进而促进L-赖氨酸的合成,目标重组菌B.subtilis XH6的L-赖氨酸产量达到(25.6±2.3) g/L。

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