鹿茸菇多糖对免疫抑制小鼠免疫及肠道菌群的作用
Effects of Lyophyllum decastes polysaccharides on immune activity and gut microbiota homeostasis in immunosuppressive mice
-
摘要: 【目的】 探究鹿茸菇多糖的免疫活性及对肠道菌群的作用。【方法】 以环磷酰胺(Cyclophosphamide,CTX)诱导免疫抑制小鼠为模型,连续20 d灌胃不同剂量的鹿茸菇多糖(Lyophyllum decastes Polysaccharide,LDPS),采用酶免疫吸附(ELISA)法、苏木精-伊红染色法、免疫印迹(WB)法和16S rDNA研究LDPS对免疫抑制小鼠胸腺和脾脏指数、血清细胞因子(白细胞介素(IL-2)和肿瘤坏死因子(TNF-α))、小肠组织分泌性免疫球蛋白A(SIgA)、脾脏和小肠组织病理形态、紧密连接蛋白及肠道菌群的影响。【结果】 LDPS可恢复免疫抑制小鼠的体质量和采食量,改善小鼠脾脏、胸腺和小肠组织损伤。与CTX模型组相比,LDPS中剂量组(MLDPS)和高剂量组(HLDPS)均能显著促进血清IL-2和小肠组织SIgA分泌,降低TNF-α含量,并显著上调小肠组织胞质紧密粘连蛋白Claudin-1(45.36%、62.89%)和闭锁小带蛋白ZO-1相对表达量(30.31%、31.35%)。各组小鼠肠道菌群存在明显差异,高剂量LDPS干预能显著提高Lachnospiraceae_NK4A136_group(93.49%)、理研菌科(Rikenellaceae)、另枝菌属(Alistipes)等有益菌的相对丰度(P<0.05),降低潜在致病菌葡萄球菌属(Staphylococcus)的相对丰度(P<0.05),逆转CTX诱导小鼠的肠道菌群紊乱并使其趋于正常。【结论】 LDPS对免疫抑制小鼠具有免疫调节作用,且其作用机制与肠道屏障和肠道菌群稳态有关。Abstract: 【Objective】 To investigate the immunomodulatory effects of Lyophyllum decastes polysaccharides(LDPS) on immune activity and gut microbiota dynamics in immunosuppressive mice. 【Methods】 An immunosuppressive mouse model was established via cyclophosphamide (CTX) administration. Following 20-day gavage of LDPS at varying doses, enzyme-linked immunosorbent assay(ELISA), hematoxylin-eosin(H&E) staining, Western blotting (WB), and 16S rDNA sequencing were employed to assess thymus/spleen indices, serum cytokines(IL-2/TNF-α), intestinal secretory immunoglobulin A(SIgA) levels, histopathological changes, tight junction protein(Claudin-1/ZO-1) expression, and gut microbiota composition. 【Results】 LDPS administration restored body mass/food intake and ameliorated splenic, thymic, and small intestinal tissue damage. Compared to the CTX model group, medium-dose(MLDPS) and high-dose(HLDPS) LDPS groups showed significantly elevated serum IL-2 levels(P<0.05) and small intestinal SIgA secretion, reduced TNF-α concentrations, and upregulated Claudin-1(45.36%/62.89% increase) and ZO-1(30.31%/31.35% increase) expression. High-dose LDPS significantly increased the relative abundance of beneficial taxa including Lachnospiraceae_NK4A136_group (93.49%), Rikenellaceae, and Alistipes(P<0.05), while decreasing potential pathogens like Staphylococcus(P<0.05), thereby reversing CTX-induced gut microbiota dysbiosis. 【Conclusion】 LDPS exhibits immunomodulatory effects in immunosuppressive mice, with mechanisms involving enhanced intestinal barrier function and restored gut microbiota homeostasis.
-
Key words:
- Lyophyllum decastes polysaccharide /
- immunity /
- gut microbiota /
- gut barrier
-
-
[1]
王碧卿.鹿茸菇的栽培现状与营养保健价值[J].食品安全导刊,2023(6):104-106. WANG B Q.The cultivation status and nutrition and health care value of Lyophyllum decastes[J].China Food Safety Magazine,2023
(6):104-106. -
[2]
李文佼,温世勇,张洪勇,等.鹿茸菇研究进展[J].中国食用菌,2022,41(3):1-5.
LI W J,WEN S Y,ZHANG H Y,et al.Research progress of Lyophyllum decastes[J].Edible Fungi of China,2022,41(3):1-5. -
[3]
贾宁.荷叶离褶伞多糖对小鼠免疫功能影响和抗氧化作用研究[C]//中国畜牧兽医学会兽医病理学分会第十六次学术研讨会、中国病理生理学会动物病理生理专业委员会第十五次学术研讨会论文集.泰安,2009:371. JIA N.Study on the effects of Lyophyllum decastes (Fr.:Fr.) Sing. polysaccharides on immune function and antioxidant activity in mice [C]//Proceedings of the 16 th Academic Symposium of the Veterinary Pathology Branch of the Chinese Association of Animal Science and Veterinary Medicine & the 15 th Academic Symposium of the Animal Pathophysiology Professional Committee of the Chinese Association of Pathophysiology.Tai’an,2009
:371. -
[4]
刘影.荷叶离褶伞菌多糖的结构鉴定及生物活性研究[D].南充:西华师范大学,2019. LIU Y.The studies on structure identification,biological activity of polysaccharide (LDS-1) from Lyophyllum decastes(Fr.:Fr.) Sing[D].Nanchong:West Normal University,2019.
-
[5]
MA Y S,WANG Q.Structural characterization of a polysaccharide from Lyophyllum decastes with MAPK-mediated immune regulation ability in mice[J].Food Science and Technology,2023,43:e003523.
-
[6]
任丽蓉,樊莹润,李泽林,等.金耳多糖对环磷酰胺诱导免疫低下小鼠的保护作用[J].食品研究与开发,2023,44(9):85-90.
REN L R,FAN Y R,LI Z L,et al.Protective effect of Tremella aurantialba polysaccharide on cyclophosphamide-induced immunocompromised mice[J].Food Research and Development,2023,44(9):85-90. -
[7]
张家豪,吕冰洁,程翔.肠道菌群通过调节免疫细胞参与肠外疾病的研究进展[J].中华内科杂志,2024,63(1):100-106.
ZHANG J H,LYU B J,CHENG X.Research progress of intestinal flora participating in extraintestinal diseases by regulating immune cells[J].Chinese Journal of Internal Medicine,2024,63(1):100-106. -
[8]
LI Y N,ZHENG J P,WANG Y,et al.Immuno-stimulatory activity of Astragalus polysaccharides in cyclophosphamide-induced immunosuppressed mice by regulating gut microbiota[J].International Journal of Biological Macromolecules,2023,242:124789.
-
[9]
HAN X,BAI B Y,ZHOU Q,et al.Dietary supplementation with polysaccharides from Ziziphus Jujuba cv.Pozao intervenes in immune response via regulating peripheral immunity and intestinal barrier function in cyclophosphamide-induced mice[J].Food & Function,2020,11(7):5992-6006.
-
[10]
刘长江,叶亚龙,孙风祥,等.环磷酰胺诱导小鼠免疫功能低下模型建立与评价[J].潍坊医学院学报,2015,37(1):4-6.
LIU C J,YE Y L,SUN F X,et al.Establishment and evaluation of immunocompromised mice model induced by cyclophosphamidein[J].Acta Academiae Medicinae Weifang,2015,37(1):4-6. -
[11]
加拿大动物管理委员会编.宋克静等译.实验用动物管理与使用指南[M].北京:原子能出版社,1993. Compiled by the Canadian Animal Management Commission.Translated by SONG K J et al.Laboratory animal management and use guidelines[M].Beijing:Atomic Energy Publishing House,1993.
-
[12]
孙思燕.荷叶离褶伞多糖结构、消化特性及结肠炎抑制作用研究[D].南京:南京农业大学,2020. SUN S Y.Study on the structure,digestive characteristics and colitis inhibition effect of polysaccharides from Lyophyllum decastes [D].Nanjing:Nanjing Agricultural University,2020.
-
[13]
李晓冰,展俊平,张月腾,等.天麻多糖对环磷酰胺所致免疫功能低下小鼠体液免疫功能的影响[J].中国老年学杂志,2016,36(5):1027-1028.
LI X B,ZHAN J P,ZHANG Y T,et al.Effect of polysaccharide from Gastrodia Elata B1 on humoral immune function in immunosuppressed mice induced by cyclophos phamide[J].Chinese Journal of Gerontology,2016,36(5):1027-1028. -
[14]
LI Q,CHEN G Y,CHEN H,et al.Se-enriched G.frondosa polysaccharide protects against immunosuppression in cyclophosphamide-induced mice via MAPKs signal transduction pathway[J].Carbohydrate Polymers,2018,196:445-456.
-
[15]
GUPTA U,HIRA S K,SINGH R,et al.Essential role of TNF-α in gamma c cytokine aided crosstalk between dendritic cells and natural killer cells in experimental murine lymphoma[J].International Immunopharmacology,2020,78:106031.
-
[16]
李钦,范强,胡继宏,等.富硒黄芪提取物对免疫抑制大鼠细胞因子IL-2、IL-4、IFN-γ及TNF-α的影响[J].西部中医药,2018,31(3):22-25.
LI Q,FAN Q,HU J H,et al.The effects of extract of selenium-enriched Astragalus membranaceus on cytokines IL-2,IL-4,IFN-γ and TNF-α of immunosuppressive rats[J].Western Journal of Traditional Chinese Medicine,2018,31(3):22-25. -
[17]
HUANG J,HUANG J L,LI Y,et al.Sodium alginate modulates immunity,intestinal mucosal barrier function,and gut microbiota in cyclophosphamide-induced immunosuppressed BALB/c mice[J].Journal of Agricultural and Food Chemistry,2021,69(25):7064-7073.
-
[18]
PIETRZAK B,TOMELA K,OLEJNIK-SCHMIDT A,et al.Secretory IgA in intestinal mucosal secretions as an adaptive barrier against microbial cells[J].International Journal of Molecular Sciences,2020,21(23):9254.
-
[19]
TANG C,DING R X,SUN J,et al.The impacts of natural polysaccharides on intestinal microbiota and immune responses:A review[J].Food & Function,2019,10(5):2290-2312.
-
[20]
汤震,顾丽霞,相兴伟,等.硒化低聚氨基多糖对免疫抑制小鼠免疫器官及肠紧密连接蛋白表达的影响[J].食品科学,2019,40(21):171-176.
TANG Z,GU L X,XIANG X W,et al.Effect of low-molecular-mass seleno-aminopolysaccharide on histomorphology and expression of tight junction proteins in immunocompromised mice[J].Food Science,2019,40(21):171-176. -
[21]
MA H,MUEED A,MA Y X,et al.Fecal microbiota transplantation activity of Floccularia Luteovirens polysaccharides and their protective effect on cyclophosphamide-induced immunosuppression and intestinal injury in mice[J].Foods,2024,13(23):3881.
-
[22]
KIM H J,LEE J S,KIM S C,et al.Immunostimulating activity of Lycium chinense Miller root extract through enhancing cytokine and chemokine production and phagocytic capacity of macrophages[J].Journal of Food Biochemistry,2020,44(6):e13215.
-
[23]
杨克铃,彭梅,杨小生,等.冬荪多糖对免疫抑制小鼠免疫功能的影响[J].食品研究与开发,2024,45(11):30-38.
YANG K L,PENG M,YANG X S,et al.Effects of polysaccharides from Phallus inpudicuon the immune function of immunosuppressed mice[J].Food Research and Development,2024,45(11):30-38. -
[24]
胡哲,王树东,董宝强,等.基于16S rDNA探讨电针对膝骨关节炎兔模型肠道菌群的影响[J].辽宁中医药大学学报,2024,26(11):89-95.
HU Z,WANG S D,DONG B Q,et al.Effect of electroacupuncture on intestinal flora of rabbit model of knee osteoarthritis based on 16S rDNA[J].Journal of Liaoning University of Traditional Chinese Medicine,2024,26(11):89-95. -
[25]
HOGENOVÁ H T,ZÁKOSTELSKÁ Z J,PETANOVÁ J,et al.Microbiota,immunity and immunologically-mediated diseases[J].Vnitrni Lekarstvi,2019,65(2):98-107.
-
[26]
WU J M,WANG S,ZHENG B,et al.Modulation of gut microbiota to enhance effect of checkpoint inhibitor immunotherapy[J].Frontiers in Immunology,2021,12:669150.
-
[27]
MAGNE F,GOTTELAND M,GAUTHIER L,et al.The firmicutes/bacteroidetes ratio:A relevant marker of gut dysbiosis in obese patients？ [J].Nutrients,2020,12(5):1474.
-
[28]
LI Z,XING J Y,MA X H,et al.An orally administered bacterial membrane protein nanodrug ameliorates doxorubicin cardiotoxicity through alleviating impaired intestinal barrier[J].Bioactive Materials,2024,37:517-532.
-
[29]
TEJADA J N,WALTERS W A,WANG Y L,et al.Prevention and cure of murine C.difficile infection by a Lachnospiraceae strain[J].Gut Microbes,2024,16(1):2392872.
-
[30]
ZHANG X S,YU D,WU D,et al.Tissue-resident Lachnospiraceae family bacteria protect against colorectal carcinogenesis by promoting tumor immune surveillance[J].Cell Host & Microbe,2023,31(3):418-432.e8.
-
[31]
ZHU Y Q,CHEN B R,ZHANG X Y,et al.Exploration of the Muribaculaceae family in the gut microbiota:Diversity,metabolism,and function[J].Nutrients,2024,16(16):2660.
-
[32]
HUANG J M,LIU D,WANG Y W,et al.Ginseng polysaccharides alter the gut microbiota and kynurenine/tryptophan ratio,potentiating the antitumour effect of antiprogrammed cell death 1/programmed cell death ligand 1 (anti-PD-1/PD-L1) immunotherapy[J].Gut,2022,71(4):734-745.
-
[33]
JI Z H,XIE W Y,ZHAO P S,et al.Kombucha polysaccharide alleviates DSS-induced colitis in mice by modulating the gut microbiota and remodeling metabolism pathways[J].Frontiers in Microbiomes,2024,3:1341824.
-
[34]
WU X C,HUANG X J,MA W N,et al.Bioactive polysaccharides promote gut immunity via different ways[J].Food & Function,2023,14(3):1387-1400.
-
[35]
PARKER B J,WEARSCH P A,VELOO A C M,et al.The genus Alistipes:Gut bacteria with emerging implications to inflammation,cancer,and mental health[J].Frontiers in Immunology,2020,11:906.
-
[36]
CAI K,CAO X Y,CHEN F,et al.Xianlian Jiedu Decoction alleviates colorectal cancer by regulating metabolic profiles,intestinal microbiota and metabolites[J].Phytomedicine,2024,128:155385.
-
[37]
TAKEUCHI T,NAKANISHI Y,OHNO H.Microbial metabolites and gut immunology[J].Annual Review of Immunology,2024,42(1):153-178.
-
[38]
张方方.鹿茸菇β-葡聚糖对肠道菌调节作用的研究[D].上海:上海应用技术大学,2023. ZHANG F F.Effect of β-glucan from Lyophyllum decastes (Fr.)Singer on gut microbiota[D].Shanghai:Shanghai Institute of Technology,2023.
-
[39]
XIANG X W,ZHENG H Z,WANG R,et al.Ameliorative effects of peptides derived from oyster (Crassostrea gigas)on immunomodulatory function and gut microbiota structure in cyclophosphamide-treated mice[J].Marine Drugs,2021,19(8):456.
-
[40]
LI Z Q,DONG J X,WANG M,et al.Resveratrol ameliorates liver fibrosis induced by nonpathogenic Staphylococcus in BALB/c mice through inhibiting its growth[J].Molecular Medicine,2022,28(1):52.
-
[1]
-
点击查看大图
计量
- PDF下载量: 2
- 文章访问数: 83
- 引证文献数: 0
下载:
