食源性致病菌PCR检测技术研究进展

胡金强; 雷俊婷; 景建洲; 孙新城; 高辉; 耿尧; 章银良; 董彩文; 姜春鹏

doi:10.3969/j.issn.2096-1553.2016.3.007

食源性致病菌PCR检测技术研究进展

郑州轻工业学院食品与生物工程学院, 河南郑州 450001;
食品生产与安全河南省协同创新中心, 河南郑州 450001;
河南省食品安全国际联合实验室, 河南郑州 450001
基金项目: 河南省高等学校青年骨干教师资助计划项目(教高[2015]1032号)
郑州轻工业学院青年骨干教师资助计划项目(2013QNGG02)
郑州轻工业学院研究生创新基金项目(2014031)
国家自然科学基金项目(31201901)
郑州轻工业学院博士基金项目(2011BSJJ033)
河南省教育厅科学技术重点研究项目(14A180025)
郑州市科技攻关项目(20130857)
教育部留学回国人员科研启动基金项目(教外司留[2013]693号)
中图分类号: TS207.4

Advance in PCR detection technologies for foodborne pathogenic bacteria

School of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China;
He'nan Collaborative Innovation Center for Food Production and Safety, Zhengzhou 450001, China;
He'nan International Joint Laboratory for Food Safety, Zhengzhou 450001, China
Received Date: 2015-05-20
Available Online: 2016-05-15
CLC number: TS207.4

摘要: 食源性致病菌是诱发食品安全问题的重要隐患,在众多食源性致病菌检测技术中,PCR检测技术因具有特异、敏感、简便、快速等优点而得以广泛应用.然而现有的包括多重PCR技术、实时荧光定量PCR技术、多重实时荧光定量PCR技术、IMS-多重实时荧光定量PCR技术、PCR-ELISA技术、EMA/PMA-PCR技术和DPO-PCR技术等在内的食源性致病菌PCR检测技术及其衍生技术仍存在成本高、效率低、质控差等缺陷,未来应向高灵敏度、高特异性、高通量、高重复性、简易、经济方向发展,以适应食源性致病菌对检测技术的要求.
- 食源性致病菌 /
- PCR检测技术 /
- 食品安全
Abstract: Foodborne pathogenic bacteria is the hidden danger of food safety.During many detection methods,PCR and PCR-derived technologies have advantages of sensible,specific,convenient and rapid,which have been widely used in the detection of foodborne pathogenic bacteria.At present,PCR detection technologies including multiple PCR,real-time PCR,multiple real-time PCR,IMS-multiplex real-time PCR,PCR-ELISA,EMA/PMA-PCR and DPO-PCR have defects such as high cost,low efficiency and poor quality control and need to be impoved to meet the requirements of high sensibility,high specificity,high throughput,high reproducibility,convenience and economics for the detection of foodborne pathogenic bacteria.
- foodborne pathogenic bacteria /
- PCR detection technology /
- food safety
1. [1]
  XU Y G,CUI L C,TIAN C Y,et al.A multiplex polymerase chain reaction coupled with high-performance liquid chromatography assay for simultaneous detection of six foodborne pathogens[J].Food control,2012,25(2):778.
2. [2]
  ⅡJIMA Y,HONDA T.Characteristics and molecular biology of verotoxin produced by enterohemorrhagic Escherichia coli.[J].Nippon rinsho,1997,55(3):646.
3. [3]
  赵玉洁.以科学精神共同应对全球挑战——2012年国际食品安全论坛在京举办[J].食品工业科技,2012,33(9):16.
4. [4]
  ABUBAKAR I,IRVINE L,ALDUS C F,et al.A systematic review of the clinical,public health and cost-effectiveness of rapid diagnostic tests for the detection and identification of bacterial intestinal pathogens in faeces and food[J].Health technology assessment,2007,11(36):1.
5. [5]
  BABU L,REDDY P,MURALI H S,et al.Optimization and evaluation of a multiplex PCR for simultaneous detection of prominent foodborne pathogens of Enterobacteriaceae[J].Annals of microbiology,2013,63(4):1591.
6. [6]
  杨晋,曾庆梅,张笛,等.添加扩增内标的沙门氏菌PCR检测方法[J].生物技术通报,2014(7):54.
7. [7]
  王丽丽,赵瑜,唐慧林,等.食品中单增李斯特菌PCR-NALF检测方法[J].食品与发酵工业,2011,37(11):194.
8. [8]
  杨胜男,郑增忍,张乐萃,等.空肠弯曲菌的培养及其PCR鉴定研究[J].动物医学进展,2012,33(12):138.
9. [9]
  WANG L X,MUSTAPHA A.EMA-Real-Time PCR as a reliable method or detection of viable Salmonella in chicken and eggs[J].Journal of food science,2010,75(3):134.
10. [10]
  郑鸣,边传周,刘仲敏.Chelex法结合环介导间接聚合酶链式反应检测肉制品单增李斯特菌[J].食品科学,2012,33(10):190.
11. [11]
  TIMMONS C,DOBHAL S,FLETCHER J,et al.Primers with 5' flaps improve the efficiency and sensitivity of multiplex PCR assays for the detection of Salmonella and Escherichia coli O157:H7[J].Journal of food protection,2013,76(4):668.
12. [12]
  CHANDRA M,CHENG P,RONDEAU G,et al.A single step multiplex PCR for identification of six diarrheagenic E.coli pathotypes and Salmonella[J].International journal of medical microbiology,2013,303(4):210.
13. [13]
  WANG Y X,ZHAO P F,ZHANG H L,et al.A simple and rapid realtime PCR assay for the detection of Shigella and Escherichia coli species in raw milk[J].Journal für verbraucherschutz und lebensmittelsicherheit,2013,8(4):313.
14. [14]
  KAO C C,LIU M F,LIN C F,et al.Antimicrobial susceptibility and multiplex PCR screening of AmpC genes from isolates of Enterobacter cloacae,Citrobacter freundii,and Serratia marcescens.[J].Journal of microbiology,immunology and infection,2010,43(3):180.
15. [15]
  魏霜,冼钰茵,赵晖,等.多重PCR检测四种食源性病原弧菌[J].中国农业科学,2013,46(8):1682.
16. [16]
  WANG L J,LI P,ZHANG Z H,et al.Rapid and accurate detection of viable Escherichia coli O157:H7 in milk using a combined IMS,sodium deoxycholate,PMA and real-time quantitative PCR process[J].Food control,2014,36(1):119.
17. [17]
  CHO M S,JOH K,AHN T Y,et al.Improved PCR assay for the specific detection and quantitation of Escherichia coli serotype O157 in water[J].Applied microbiology and biotechnology,2014,98(18):7869.
18. [18]
  LEE J L,LEVIN R E.Detection of 5 CFU/g of Escherichia coli O157:H7 on lettuce using activated charcoal and real-time PCR without enrichment[J].Food microbiology,2011,28(3):562.
19. [19]
  杜雄伟,李叶,江洁,等.肉制品中沙门氏菌invA基因实时荧光定量PCR检测方法的建立[J].食品工业科技,2013,34(12):68.
20. [20]
  邵美丽,许岩,刘思国,等.TaqMan探针实时定量PCR检测肉中金黄色葡萄球菌的研究[J].食品工业,2013,34(3):109.
21. [21]
  周慧,支竹伟,刘涵,等.多重实时荧光定量PCR检测肴肉中的特定腐败菌[J].粮食与食品工业,2014,21(3):86.
22. [22]
  CREMONESI P,PISANI L F,LECCHI C,et al.Development of 23 individual TaqMan^® real-time PCR assays for identifying common foodborne pathogens using a single set of amplification conditions[J].Food microbiology,2014,43:35.
23. [23]
  GORDILLO R,RODRIGUEZ A,WERNING M,et al.Quantification of viable Escherichia coli O157:H7 in meat products by duplex real-time PCR assays[J].Meat science,2014,96(2):964.
24. [24]
  KÖPPEL R,KUSLYTE A R,TOLIDO I,et al.Nonaplex real-time PCR detection of Listeria monocytogenes,Campylobacter,Salmonella and enteropathogene E.coli after universal enrichment in food samples[J].European food research and technology,2013,237(3):315.
25. [25]
  邵美丽,董鑫,赵燕丽,等.单增李斯特菌和金黄色葡萄球菌双重荧光定量PCR检测方法建立[J].食品科学,2013,34(16):169.
26. [26]
  索标,滕要辉,艾志录,等.食源性致病菌多重荧光PCR检测扩增内标的构建及评价[J].食品与发酵工业,2011,37(8):148.
27. [27]
  GUY R A,TREMBLAY D,BEAUSOLEIL L,et al.Quantification of E.coli O157 and STEC in feces of farm animals using direct multiplex real time PCR(qPCR) and a modified most probable number assay comprised of immunomagnetic bead separation and qPCR detection[J].Journal of microbiological methods,2014,99:44.
28. [28]
  马凯,李宝明,白羽,等.基于免疫磁分离的三重荧光定量PCR检测食品中沙门氏菌、志贺氏菌和金黄色葡萄球菌[J].微生物学通报,2014,41(11):2369.
29. [29]
  BARANZONI G M,FRATAMICO P M,RUBIO F,et al.Detection and isolation of Shiga toxin-producing Escherichia coli(STEC) O104 from sprouts[J].International journal of food microbiology,2014,173:99.
30. [30]
  闻一鸣,李志清,童吉宇,等.免疫磁珠富集技术联合选择性培养基快速检测单增李斯特菌[J].生物工程学报,2012,29(5):672.
31. [31]
  胡金强,雷俊婷,詹丽娟,等.免疫学技术在食源性微生物检测中的应用综述[J].郑州轻工业学院(自然科学版),2014,29(3):7.
32. [32]
  DELBEKE S,CEUPPENS S,HOLVOET K,et al.Multiplex real-time PCR and culture methods for detection of Shiga toxin-producing Escherichia coli and Salmonella Thompson in strawberries,a lettuce mix and basil[J].International journal of food microbiology,2015,193:1.
33. [33]
  Rebecca AG,Donald T,Louise B,et al.Quantification of E. coli O157 and STEC in feces of farm animals using direct multiplex real time PCR (qPCR) and a modified most probable number assay comprised of immunomagnetic bead separation and qPCR detection[J].Journal of microbiological methods,2014,99:44.
34. [34]
  谭炳乾,何启盖,肖军,等.建立PCR-ELISA方法检测单核细胞增多性李斯特菌[J].农业生物技术学报,2008,16(4):670.
35. [35]
  王丹,刘金华,史艳宇,等.大肠杆菌O157:H7 PCR-ELISA检测方法的建立[J].黑龙江畜牧兽医,2014(6):128.
36. [36]
  LI Y H,CAO L,ZHANG C,et al.Development and evaluation of a PCR-ELISA assay for the detection and quantification of Cronobacter spp.[J].International dairy journal,2013,33(1):27.
37. [37]
  谭炳乾,何启盖,肖军,等.建立PCR-ELISA方法检测单核细胞增多性李斯特菌[J].农业生物技术学报,2008,16(4):670.
38. [38]
  PERELLE S,DILASSER F,GROUT J,et al.Screening food raw materials for the presence of the world's most frequent clinical cases of Shiga toxin-encoding Escherichia coli O26,O103,O111,O145 and O157[J].International journal of food microbiology,2007,113(3):284.
39. [39]
  史艳宇,刘金华,薛力刚,等.PCR-ELISA法检测食品中空肠弯曲菌[J].食品科学,2013,34(10):246.
40. [40]
  ELIZAQUIVEL P,SANCHEZ G,AZNAR R.Quantitative detection of viable foodborne E.coli O157:H7,Listeria monocytogenes and Salmonella in fresh-cut vegetables combining propidium monoazide and real-time PCR[J].Food control,2012,25(2):704.
41. [41]
  丁林贤,苏晓梅,横田明.活的但非可培养(VBNC)状态菌的研究进展[J].微生物学报,2011,51(7):858.
42. [42]
  DINU L D,BACH S.Detection of viable but non-culturable Escherichia coli O157:H7 from vegetable samples using quantitative PCR with propidium monoazide and immunological assays[J].Food control,2013,31(2):268.
43. [43]
  高延玲,狄元冉,李金磊,等.EMA与PCR结合检测大肠杆菌O157方法的研究[J].畜牧与饲料科学,2014,35(10):9.
44. [44]
  胡金强,雷俊婷,詹丽娟,等.食源性微生物的分子生物学检测方法的研究进展[J].食品工业,2014,35(7):201.
45. [45]
  刘艳艳,柳增善,卢士英,等.灭菌乳中活阪崎肠杆菌PMA-PCR检测方法的建立[J].中国畜牧兽医,2014,41(2):65.
46. [46]
  Wang L J,Li P,Zhang Z H,et al.Rapid and accurate detection of viable Escherichia coli O157:H7 in milk using a combined IMS,sodium deoxycholate,PMA and real-time quantitative PCR process[J].Food Control,2014,36:119.
47. [47]
  THIERRY S,HAMIDJAJA R A,GIRAULT G,et al.A multiplex bead-based suspension array assay for interrogation of phylogenetically informative single nucleotide polymorphisms for Bacillus anthracis[J].Journal of microbiological methods,2013,95(3):357.
48. [48]
  徐义刚,李丹丹,崔丽春,等.应用DPO-PCR技术检测肠出血性大肠杆菌O157:H7[J].食品科学,2014,35(8):160.
49. [49]
  XU Y G,LI D D,ZHANG B Q,et al.Dual-priming primers-based PCR method for specific detection of Listeria monocytogenes[J].Science and technology of food industry,2014,35(10):86.
50. [50]
  KIM J K,LEE H J,LEE Y J,et al.Direct detection of lamivudine-resistant hepatitis B virus mutants by a multiplex PCR using dual-priming oligonucleotide primers[J].Journal of virological methods,2008,149(1):76.
51. [51]
  徐义刚,李丹丹,刘忠梅,等.应用DPO-PCR方法特异性检测志贺氏菌[J].中国畜牧兽医,2014,41(3):28.
1. [1]
  李跑 , 谭惠珍 , 谢叔娥 , 苏光林 , 董怡青 , 唐辉 . 基于近红外光谱技术有监督模式识别的青皮产地溯源分析. 轻工学报, 2024, 0(0): -.

点击查看大图

计量

PDF下载量: 129
文章访问数: 8442
引证文献数: 0

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

食源性致病菌PCR检测技术研究进展

Advance in PCR detection technologies for foodborne pathogenic bacteria

计量

通讯作者: 陈斌, bchen63@163.com

食源性致病菌PCR检测技术研究进展

English Abstract

Advance in PCR detection technologies for foodborne pathogenic bacteria

目录

食源性致病菌PCR检测技术研究进展

Advance in PCR detection technologies for foodborne pathogenic bacteria

计量

文章相关

通讯作者: 陈斌, bchen63@163.com

食源性致病菌PCR检测技术研究进展

English Abstract

Advance in PCR detection technologies for foodborne pathogenic bacteria

目录